Compression of the trigeminal ganglion produces prolonged nociceptive behavior in rats
Compression of the trigeminal ganglion produces prolonged nociceptive behavior in rats
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DOI：
10.1016/j.ejpain.2008.07.008
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发表时间：
2009-07-01
影响因子：
3.6
通讯作者：
Bae, Yong C.
中科院分区：
文献类型：
--
作者：
Ahn, Dong K.;Lim, Eun J.;Bae, Yong C.
本研究首次证实了大鼠三叉神经节压迫后三叉神经区的伤害性行为延长。在戊巴比妥钠麻醉下，在固定在立体定位架上的雄性Sprague-Dawley大鼠上进行实验。为了压迫三叉神经节，通过不锈钢注射器（24号）将4%琼脂溶液（8 μ l）注射到三叉神经节中，该注射器延伸超过引导套管（21号）的端部2 mm。琼脂注射后，取出注射器和导引套管。在对照组中，大鼠假手术不注射琼脂。在手术前3天和手术后3、7、10、14、17、21、24、30和40天检查吹气阈值（机械性异常性疼痛）、针刺反应（机械性痛觉过敏）和自发性抓挠行为。使用重复测量ANOVA分析数据，然后使用LSD事后检验进行多组比较。压迫三叉神经节后，喷气阈值显著降低。机械性异常性疼痛在3天内建立，并持续到术后第24天。机械性痛觉过敏在压迫后3天也很明显，并持续到术后第40天。虽然机械异常性疼痛和痛觉过敏出现双边，同侧是显着更敏感。腹腔注射卡马西平可显著阻断三叉神经节压迫引起的机械性异常性疼痛。这些发现表明，在该动物模型中，压迫三叉神经节后的长时间伤害性行为可能与三叉神经痛相似。(C)2008年，国际疼痛研究协会欧洲分会联合会。由爱思唯尔有限公司出版。保留所有权利。
The present study is the first demonstration of prolonged nociceptive behavior in the trigeminal region following compression of the trigeminal ganglion in rats. Experiments were carried out on male Sprague-Dawley rats mounted onto a stereotaxic frame under pentobarbital sodium anesthesia. For compression of the trigeminal ganglion, a 4% agar solution (8 mu l) was injected into the trigeminal ganglion through a stainless steel injector (24 gauge), which extended 2 mm beyond the end of a guide cannula (21 gauge). Following agar injection, the injector and guide cannula were removed. In the control group, rats were sham operated without agar injection. Air-puff thresholds (mechanical allodynia), pin prick responses (mechanical hyperalgesia), and spontaneous scratching behavior were examined 3 days before surgery and at 3, 7, 10, 14, 17, 21, 24, 30, and 40 days after surgery. Data were analyzed using a repeated measures ANOVA followed by multiple group comparisons using the LSD post-hoc test. Air-puff thresholds significantly decreased after compression of the trigeminal ganglion. Mechanical allodynia was established within 3 days and lasted beyond postoperative day 24. Mechanical hyperalgesia was also evident 3 days after compression and persisted until the 40th postoperative day. Although mechanical allodynia and hyperalgesia appeared bilaterally, the ipsilateral side was significantly more sensitive. Intraperitoneal treatment with carbamazepine significantly blocked mechanical allodynia produced by compression of the trigeminal ganglion. These findings suggest that prolonged nociceptive behavior following compression of the trigeminal ganglion may mimic trigeminal neuralgia in this animal model. (C) 2008 European Federation of Chapters of the International Association for the Study of Pain. Published by Elsevier Ltd. All rights reserved.