Lactobacillus acidophilus Increases the Anti-apoptotic Micro RNA-21 and Decreases the Pro-inflammatory Micro RNA-155 in the LPS-Treated Human Endothelial Cells
Lactobacillus acidophilus Increases the Anti-apoptotic Micro RNA-21 and Decreases the Pro-inflammatory Micro RNA-155 in the LPS-Treated Human Endothelial Cells
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DOI：
10.1007/s12602-016-9214-1
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发表时间：
2016-06-01
影响因子：
4.9
通讯作者：
Doroudchi, Mehrnoosh
中科院分区：
文献类型：
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作者：
Kalani, Mehdi;Hodjati, Hossein;Doroudchi, Mehrnoosh
鉴于益生菌在动脉粥样硬化中的抗炎和保护作用以及微RNA （miRNA）对内皮细胞（dys）功能的调节作用，本研究旨在研究嗜酸乳杆菌（La）对大肠杆菌脂多糖（Ec-LPS）诱导的人脐静脉内皮细胞（HUVEC）细胞死亡及miRNA-21、92a、155和663表达的影响。lps处理和未处理的HUVECs分别在不同的La条件下培养，如La条件培养基（LaCM）、La水提物（LaWE）、La培养过滤（LaFS）和未过滤的上清（LaUFS）。24h后，分别用流式细胞术和real-time PCR方法检测细胞凋亡、坏死和上述mirna的水平。在ec - lps处理的huvec中，LaCM减少凋亡、坏死和炎症性miR-155，相反增加抗凋亡miR-21。关联分析显示坏死与miR-21、miR-92a和miR-155水平呈负相关。嗜酸乳杆菌对内皮细胞死亡和动脉粥样硬化相关mirna在这些细胞中的表达的有益作用暗示了其在心血管疾病调节中的一个新方面，而不是之前所描述的，并表明这种益生菌是动脉粥样硬化预防性治疗的候选菌。
Given the anti-inflammatory and protective role of probiotics in atherosclerosis and the regulatory role of micro RNA (miRNA) in endothelial cell (dys) functions, this study aimed to investigate the effect of Lactobacillus acidophilus (La) on cellular death and the expression of miRNA-21, 92a, 155, and 663 in human umbilical vein endothelial cell (HUVEC) induced by Escherichia coli lipopolysaccharide (Ec-LPS). LPS-treated and untreated HUVECs were cultured in the presence of different La conditions such as La-conditioned media (LaCM), La water extract (LaWE), La culture-filtered (LaFS) and unfiltered supernatants (LaUFS). After 24 h, apoptosis, necrosis and the levels of the mentioned miRNAs were measured using flow cytometry and real-time PCR methods, respectively. LaCM decreased apoptosis, necrosis and inflammatory miR-155 and conversely increased antiapoptotic miR-21 in Ec-LPS-treated HUVECs. Association analysis revealed negative correlations between necrosis and the levels of miR-21, miR-92a, and miR-155. The beneficial effects of L. acidophilus on the ECs death and expression of atherosclerosis related miRNAs in these cells imply a new aspect of its regulation in cardiovascular diseases rather than previously described ones and suggest this probiotic bacterium as a candidate in the preventative therapy of atherosclerosis.