Emerging techniques in single-cell epigenomics and their applications to cancer research.
Emerging techniques in single-cell epigenomics and their applications to cancer research.
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DOI：
10.4172/jcg.1000103
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zhou Q
中科院分区：
文献类型：
--
作者：
Lo PK;Zhou Q
表观基因组学包括研究基因组DNA和相关组蛋白的化学修饰，基因组DNA序列和蛋白质之间的相互作用，染色体构象的动态，这些表观遗传事件之间的功能关系，以及这些表观遗传事件对细胞中基因表达的调控影响。与目前只能表征整个细胞系的平均表观基因组特征的技术相比，单细胞表观基因组方法正在成为研究细胞可塑性和异质性的强大新技术，如在干细胞和癌症中所见。在这里，我们总结了单细胞表观基因组学研究的可用技术，审查其目前的应用于癌症研究，并讨论未来的可能性。通过整合基因组学、表观基因组学和转录组学的多组学信息，可以充分发挥单细胞表观遗传学研究的潜力。
Epigenomics encompasses studies of the chemical modifications of genomic DNA and associated histones, interactions between genomic DNA sequences and proteins, the dynamics of the chromosomal conformation, the functional relationships between these epigenetic events, and the regulatory impacts of these epigenetic events on gene expression in cells. In comparison to current techniques that are only capable of characterizing average epigenomic features across bulk cell ensembles, single-cell epigenomic methodologies are emerging as powerful new techniques to study cellular plasticity and heterogeneity, as seen in stem cells and cancer. Here we summarize available techniques for studies of single-cell epigenomics, review their current applications to cancer research, and discuss future possibilities. This review also highlights that the full potential of single-cell epigenetic studies will be comprehended through integrating the multi-omics information of genomics, epigenomics and transcriptomics.