Novel regional and developmental NMDA receptor expression patterns uncovered in NR2C subunit-β-galactosidase knock-in mice
Novel regional and developmental NMDA receptor expression patterns uncovered in NR2C subunit-β-galactosidase knock-in mice
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DOI：
10.1016/j.mcn.2006.12.001
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发表时间：
2007-03-01
影响因子：
3.5
通讯作者：
Buonanno, Andres
中科院分区：
文献类型：
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作者：
Karavanova, Irina;Vasudevan, Kuzhalini;Buonanno, Andres
通过在NR2C亚基翻译起始位点插入核p -半乳糖苷酶报告基因产生NMDA受体“敲入”小鼠。在这些小鼠中发现了新的细胞类型和NR2C表达的动态模式，这在以前是不被注意到的，因为特异性识别含有NR2C受体的试剂不存在。我们在连接脾后皮层和海马的区域发现了一个从表达nr2c的神经元到星形胶质细胞的过渡区。我们证明NR2C在条纹、嗅球和大脑皮层的s10013阳性/ gfap阴性胶质细胞亚群中表达。我们还展示了新的神经元表达区域，如脾后皮层、丘脑、脑桥和前庭核。此外，我们发现，在小脑发育过程中，NR2C在位于内颗粒层的颗粒ceu亚群的瞬时尾吻梯度和副矢状带中表达，进一步证明了颗粒神经元的异质性。这些结果表明含有nr2c的NMDA受体具有新的功能。(c) 2006爱思唯尔公司版权所有。
NMDA receptor "knock-in" mice were generated by inserting the nuclear P-galactosidase reporter at the NR2C subunit translation initiation site. Novel cell types and dynamic patterns of NR2C expression were identified using these mice, which were unnoticed before because reagents that specifically recognize NR2C-containing receptors are nonexistent. We identified a transition zone from NR2C-expressing neurons to astrocytes in an area connecting the retrosplenial cortex and hippocampus. We demonstrate that NR2C is expressed in a subset of S10013-positive/GFAP-negative glial cells in the striation, olfactory bulb and cerebral cortex. We also demonstrate novel areas of neuronal expression such as retrosplenial cortex, thalamus, pontine and vestibular nuclei. In addition, we show that during cerebellar development NR2C is expressed in transient caudal-rostral gradients and parasagittal bands in subsets of granule ceUs residing in the internal granular layer, further demonstrating heterogeneity of granule neurons. These results point to novel functions of NR2C-containing NMDA receptors. (c) 2006 Elsevier Inc. All rights reserved.