Marked effect of RhoA-specific shRNA-producing plasmids on neurite growth in PC12 cells
Marked effect of RhoA-specific shRNA-producing plasmids on neurite growth in PC12 cells
复制标题
RhoA 特异性 shRNA 产生质粒对 PC12 细胞神经突生长的显着影响
DOI：
10.1016/j.neulet.2008.05.045
复制
发表时间：
2008-08-01
影响因子：
2.5
通讯作者：
Cui, Qi
中科院分区：
文献类型：
--
作者：
Fan, You-Ming;Pang, Chi-Pui;Cui, Qi
为了促进神经突生长，我们构建了一种产生RhoA特异性小发夹RNA （shRNA）的质粒来阻断RhoA的表达，并在PC12细胞中测试了其作用。结果表明，shrna介导的RNA干扰（RNAi）成功抑制了RhoA的表达。由于RhoA敲低，f -肌动蛋白水平显著降低，过程明显诱导。这些过程表达两种神经突标记物：神经丝和小管蛋白。本研究表明，质粒产生RhoA特异性shRNA可以作为诱导神经突生长的有效工具，进一步证实了RhoA对神经突生长的抑制作用。因此，这种shRNA可能是促进神经突起生长的有用工具，可用于中枢神经系统损伤后的神经修复。2008爱思唯尔爱尔兰有限公司版权所有。
In order to promote neurite outgrowth, we constructed a plasmid producing RhoA-specific small hairpin RNA (shRNA) to block RhoA expression and tested its actions in PC12 cells. Our results show that the shRNA-mediated RNA interference (RNAi) successfully suppressed RhoA expression. As a consequence of RhoA knockdown, F-actin levels were significantly reduced and processes were markedly induced. These processes express two neurite markers, neurofilament and Pill tubulin. This study demonstrates that plasmid-producing shRNA specific for RhoA can act as an effective tool to induce neurite outgrowth and further confirms the neurite growth-inhibitory role of RhoA. This shRNA may thus be a useful tool in promoting neurite outgrowth and could be applicable in neural repair after CNS injury. (c) 2008 Elsevier Ireland Ltd. All rights reserved.