Variation of Microsatellite Size Homoplasy Across Electromorphs, Loci, and Populations in Three Invertebrate Species
Variation of Microsatellite Size Homoplasy Across Electromorphs, Loci, and Populations in Three Invertebrate Species
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DOI：
10.1007/pl00006361
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发表时间：
1998-07
影响因子：
3.9
通讯作者：
F. Viard;P. Franck;M. Dubois;A. Estoup;P. Jarne
中科院分区：
文献类型：
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作者：
F. Viard;P. Franck;M. Dubois;A. Estoup;P. Jarne
通过对电形态（即相同大小（碱基对）的变体）进行测序，分析了微卫星位点的大小同质性。这项研究使用三种无脊椎动物物种（蜜蜂Apis mellifera、熊蜂Bombus terrestris 和淡水蜗牛Bulinus truncatus）的五个中断和/或复合基因座进行。测序的 15 个电形态隐藏了 31 个等位基因（即序列相同的变体）。在电形态和基因座之间检测到大小同质性的变化。每个电形态检测到一到七个等位基因，并且两个蜂种中的一个基因座没有显示任何大小同质性。大小同质性的量与测序工作相关，因为等位基因的数量与测序的电形态的拷贝数相关，而且与每个基因座的核心序列的分子结构相关。群体内的尺寸同质性仅检测到 3 次，这意味着尺寸同质性主要在群体中检测到。我们分析了种群结构，根据电形态或等位基因估计了遗传距离。而 A 中几乎没有发现差异。 mellifera，发现大小同质性导致 B 中更明显的种群结构。陆地 B. truncatus。我们还在A中展示了。人们认为尺寸同质性的检测可能会改变系统发育重建。
Size homoplasy was analyzed at microsatellite loci by sequencing electromorphs, that is, variants of the same size (base pairs). This study was conducted using five interrupted and/or compound loci in three invertebrate species, the honey beeApis mellifera,the bumble beeBombus terrestris,and the freshwater snailBulinus truncatus.The 15 electromorphs sequenced turned out to hide 31 alleles (i.e., variants identical in sequence). Variation in the amount of size homoplasy was detected among electromorphs and loci. From one to seven alleles were detected per electromorph, and one locus did not show any size homoplasy in both bee species. The amount of size homoplasy was related to the sequencing effort, since the number of alleles was correlated with the number of copies of electromorphs sequenced, but also with the molecular structure of the core sequence at each locus. Size homoplasy within populations was detected only three times, meaning that size homoplasy was detected mostly among populations. We analyzed population structure, estimatingFstand a genetic distance, based on either electromorphs or alleles. Whereas little difference was found inA. mellifera,uncovering size homoplasy led to a more marked population structure inB. terrestrisandB. truncatus.We also showed inA. melliferathat the detection of size homoplasy may alter phylogenetic reconstructions.