Nanopillared Surfaces Disrupt Pseudomonas aeruginosa Mechanoresponsive Upstream Motility.
Nanopillared Surfaces Disrupt Pseudomonas aeruginosa Mechanoresponsive Upstream Motility.
复制标题
DOI：
10.1021/acsami.8b22262
复制
发表时间：
2019-02
影响因子：
9.5
通讯作者：
Rachel Rosenzweig;K. Perinbam;V. K. Ly;Siavash Ahrar;Albert Siryaporn;A. Yee
中科院分区：
文献类型：
--
作者：
Rachel Rosenzweig;K. Perinbam;V. K. Ly;Siavash Ahrar;Albert Siryaporn;A. Yee
铜绿假单胞菌是一种机会性、多重耐药的人类病原体，其在具有流体流动的环境中形成生物膜，例如囊性纤维化患者的肺、工业管道和医疗设备。铜绿假单胞菌通过其机械响应IV型皮利运动附件的周期性伸展和收缩在表面上向上游抽搐。在流体流动系统中防止上游运动性、宿主入侵和感染性生物膜形成仍然是未满足的挑战。在这里，我们描述了可扩展的纳米柱表面结构的设计和应用，制造使用纳米压印光刻，减少上游的运动和铜绿假单胞菌的殖民。我们使用流动通道来诱导通常在导管中发现的剪切应力，并进行显微镜分析，以研究具有不同填充分数的纳米柱表面对上游运动轨迹、位移、速度和表面附着的影响。我们发现，密集的，亚细胞纳米柱表面，柱周期性范围从200至600 nm和宽度范围从70至215 nm，抑制机械响应上游运动和表面附着。这种细菌-纳米结构表面界面效应使我们能够定制具有特定纳米柱几何形状的表面，用于破坏流体流动系统中的细胞运动和附着。
Pseudomonas aeruginosa is an opportunistic, multidrug-resistant, human pathogen that forms biofilms in environments with fluid flow, such as the lungs of cystic fibrosis patients, industrial pipelines, and medical devices. P. aeruginosa twitches upstream on surfaces by the cyclic extension and retraction of its mechanoresponsive type IV pili motility appendages. The prevention of upstream motility, host invasion, and infectious biofilm formation in fluid flow systems remains an unmet challenge. Here, we describe the design and application of scalable nanopillared surface structures fabricated using nanoimprint lithography that reduce upstream motility and colonization by P. aeruginosa. We used flow channels to induce shear stress typically found in catheter tubes and microscopy analysis to investigate the impact of nanopillared surfaces with different packing fractions on upstream motility trajectory, displacement, velocity, and surface attachment. We found that densely packed, subcellular nanopillared surfaces, with pillar periodicities ranging from 200 to 600 nm and widths ranging from 70 to 215 nm, inhibit the mechanoresponsive upstream motility and surface attachment. This bacteria-nanostructured surface interface effect allows us to tailor surfaces with specific nanopillared geometries for disrupting cell motility and attachment in fluid flow systems.