SPONTANEOUS LEUKOCYTE ROLLING IN VENULES IN UNTRAUMATIZED SKIN OF CONSCIOUS AND ANESTHETIZED ANIMALS
SPONTANEOUS LEUKOCYTE ROLLING IN VENULES IN UNTRAUMATIZED SKIN OF CONSCIOUS AND ANESTHETIZED ANIMALS
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DOI：
10.1152/ajpheart.1994.267.3.h1199
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发表时间：
1994-09-01
影响因子：
--
通讯作者：
RENEMAN, RS
中科院分区：
文献类型：
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作者：
JANSSEN, GHGW;TANGELDER, GJ;RENEMAN, RS
活体明视野视频显微镜被用来研究是否可以在正常生理条件下观察到白细胞滚动。我们研究了训练清醒的大鼠和麻醉的大鼠和小鼠的皮肤微静脉，而不接触皮肤本身。在刘易斯大鼠（直径8-27 μ m）的所有后爪小静脉和所有小鼠耳小静脉（Swiss，13-38 μ m; BALB/c，12-56 μ m）中自发存在白细胞滚动。清醒大鼠的滚动水平（白细胞/分钟，中位数和范围）为8（3-15），麻醉大鼠为9（3-19），麻醉瑞士小鼠为30（5-160），麻醉BALB/c小鼠为10（3-22）。这些水平似乎与时间无关。非侵入性机械刺激诱导平均增加32%。用吖啶橙子荧光标记体内白细胞没有影响。在瑞士小鼠中，> 75%的白细胞滚动速度< 50 μ m/s（中位数为31 μ m/s）;该参数与降低的速度（17-68 s（-1））无关，因此与壁剪切率无关。我们的发现，白细胞滚动是自发地存在于皮肤静脉麻醉和清醒的动物表明，在皮肤中的主机防御机制的持续警惕。
Intravital bright-field videomicroscopy was used to investigate whether leukocyte rolling can be observed under normal physiological conditions. We studied skin venules in trained conscious rats and anesthetized rats and mice without touching the skin itself. Leukocyte rolling was spontaneously present in all hindpaw venules of Lewis rats (diam 8-27 mu m) and also in all mouse ear venules (Swiss, 13-38 mu m; BALB/c, 12-56 mu m). Rolling levels (in leukocytes/min, median and range) were 8 (3-15) in conscious rats, 9 (3-19) in anesthetized rats, 30 (5-160) in anesthetized Swiss mice, and 10 (3-22) in anesthetized BALB/c mice. These levels appeared to be independent of time. Noninvasive mechanical stimulation induced an average increase of 32%. Fluorescent labeling of leukocytes in vivo with acridine orange had no influence. In Swiss mice, the rolling velocity was < 50 mu m/s for > 75% of the leukocytes (median 31 mu m/s); this parameter did not correlate with reduced velocity (17-68 s(-1)) and hence wall shear rate. Our finding that leukocyte rolling is spontaneously present in skin venules of anesthetized and conscious animals suggests a constant vigilance of the host defense mechanisms in the skin.