The fates of different types of adipose tissue after transplantation in mice
The fates of different types of adipose tissue after transplantation in mice
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小鼠移植后不同类型脂肪组织的命运。
DOI：
10.1096/fj.202200408r
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发表时间：
2022-09-01
期刊：
影响因子：
4.8
通讯作者：
Cai, Junrong
中科院分区：
文献类型：
--
作者：
Jiang, Shenglu;Lin, Jiayan;Cai, Junrong
脂肪移植是最常用的软组织修复方法之一。然而，目前尚不清楚脂肪组织的类型是否会对脂肪移植物的存活产生任何影响。本研究旨在确定三种不同类型的脂肪组织的脂肪移植的命运。本研究将小鼠随机分为白色脂肪组织（WAT）组、米色脂肪组织（beigeAT）组和棕色脂肪组织（BAT）组。移植前，供体小鼠注射罗格列酮或磷酸盐缓冲盐水（PBS）。WAT和BAT获自PBS处理的小鼠，而米色AT获自罗格列酮处理的小鼠。将三种类型的脂肪组织（各150 mg）分别移植到三组中，并在2、4或12周时收获。BAT和米色AT含有较小的脂肪细胞，并表达较高水平的解偶联蛋白-1基因。移植脂肪的留存率对于米色脂肪显著高于对于白色脂肪，但是对于棕色脂肪显著低于对于白色脂肪。移植的棕色脂肪的特点是上调炎症和高内质网应激。相比之下，米色AT组的脂肪移植物显示出最好的成脂能力、中等的炎症水平和良好的上级血管生成。在体外缺氧条件下，米色脂肪细胞组的凋亡细胞数少于棕色和白色脂肪细胞组。棕色脂肪细胞的条件培养基可诱导RAW 264.7巨噬细胞的M1极化，而米色脂肪细胞的条件培养基可有效促进M2极化。因此，我们认为米色AT由于低炎症和上级存活率而为脂肪移植提供了一种新的潜在选择，但BAT由于其存活率差而可能不是脂肪移植的理想选择。
Fat grafting is one of the most commonly applied procedure for soft-tissue repair. However, it remains unclear whether the type of adipose tissue would have any effects on fat graft survival. The present study aimed to determine fates of fat grafting of three different types of fat tissue. In this study, mice were randomly divided into three groups, white adipose tissue (WAT) group, beige adipose tissue (beige AT) group and brown adipose tissue (BAT) group. Before transplantation, donor mice were injected with rosiglitazone or phosphate-buffered saline (PBS). The WAT and BAT were obtained from PBS-treated mice while beige AT was obtained from the rosiglitazone-treated mice. Three types of fat tissue (150 mg each) were transplanted in three groups, respectively, and harvested at 2, 4 or 12 weeks. The BAT and beige AT contained smaller adipocytes and expressed higher level of uncoupling protein-1 gene. The retention rate of the transplanted fat was significantly higher for beige than for white fat, but was significantly lower for brown than for white fat. Transplanted brown fat was characterized by upregulated inflammation and high endoplasmic reticulum stress. By contrast, fat grafts in beige AT group showed the best adipogenic capacity, moderate inflammation level and superior angiongenesis. In vitro, under hypoxic condition, fewer apoptotic cells were found in beige adipocyte group than that in brown and white adipocyte group. Conditioned medium from brown adipocytes induced M1 polarization of RAW 264.7 macrophages while that from beige adipocytes effectively promoted M2 polarization. Therefore, we suggest that beige AT provides a new potential choice for fat grafting because of low inflammation and superior survival but BAT might not be ideal for fat grafting due to its poor survival.