Xylose-DNA: comparison of the thermodynamic stability of oligo(2'-deoxyxylonucleotide) and oligo(2'-deoxyribonucleotide) duplexes.
Xylose-DNA: comparison of the thermodynamic stability of oligo(2'-deoxyxylonucleotide) and oligo(2'-deoxyribonucleotide) duplexes.
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木糖-DNA：寡聚（2-脱氧木核苷酸）和寡聚（2-脱氧核糖核苷酸）双链体的热力学稳定性比较。
DOI：
10.1111/j.1432-1033.1996.0033u.x
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
F. Seela
中科院分区：
文献类型：
--
作者：
Arnulf Schöppe;Hans;H. Rosemeyer;F. Seela
对合成的两个寡聚(2‘-脱氧核苷酸)：(A)d[xA)3-(XT)3-(XA)3-(XT)3-T]和(B)d[(xA-xT)6-T]和寡聚(2’-脱氧核苷酸)(C)d[(A)3-(T)3-(A)3-(T)3]进行了差示扫描量热、紫外吸收和圆二色谱测量。以2‘-脱氧核糖取代2’-脱氧核糖的寡核苷酸具有独特的热力学、光学和结构特征。在相同浓度下，低聚物(2‘-脱氧核寡聚物)的转变温度高于低聚物(2’-脱氧核糖低聚物)，表明其稳定性较高。(C)的量热转变热为270+/-15kJ。摩尔-1，对应的范特霍夫值为280+/-15kJ。(合作单位摩尔)-1。增量Hh/增量Hcal=1.04的比值表明，2‘-脱氧核糖寡核苷酸的转变是全有或全不的行为。(A)的相似参数为：ΔHcal=310+/-30kJ。摩尔-1，增量HVH=220+/-30kJ(合作单元摩尔)-1。0.71的比率表明该化合物处于多态熔融状态。当交替的寡聚(2‘-脱氧核苷酸)d[(xA-xT)6-T]的参数与d[(XA)3-(XT)3-(XA)3-(XT)3-T)的参数进行比较时，热力学参数的顺序依赖性变得明显。其取值为：增量Hcal=330+/-30kJ.mol-1，增量Hh=180+/-15kJ(摩尔单位摩尔)-1，增量Hcal/增量Hcal=0.55。在所研究的寡核苷酸中，交替低聚(2‘-脱氧核苷酸)(B)的转变热最高，但转变的协同性最低。两个寡核苷酸(2‘-脱氧核苷酸)的圆二色谱显示出不寻常的特征，d[(XA)3-(XT)3-(XA)3-(XT)3-T]表现出一种左旋双螺旋的光谱，而交替的寡(2’-脱氧核苷酸)(B)的光谱既不像(C)，也不像(A)。
Measurements of differential scanning calorimetry, ultraviolet absorption and circular dichroism have been performed on two synthetic oligo(2'-deoxyxylonucleotides): (A) d[xA)3-(xT)3-(xA)3-(xT)3-T] and (B) d[(xA-xT)6-T], and on the oligo(2'-deoxyribonucleotide) (C) d[(A)3-(T)3-(A)3-(T)3]. Oligonucleotides having 2'-deoxyxylose instead of 2'-deoxyribose exhibit unusual thermodynamic, optical and structural features. At identical concentrations the transition temperatures of the oligo(2'-deoxyxylooligomers) are higher than those of the oligo(2'-deoxyribooligomers) indicating higher stability. The calorimetric transition enthalpy of (C) is 270 +/- 15 kJ . mol-1, the corresponding van't Hoff value is 280 +/- 15 kJ . (mol of cooperative unit)-1. The ratio of delta HvH/delta Hcal = 1.04 suggests all-or-none behaviour for the transition of the 2'-deoxyribose oligonucleotide. The analogous parameters of (A) are: delta Hcal = 310 +/- 30 kJ . mol-1, delta HvH = 220 +/- 30 kJ.(mol of cooperative unit)-1. The ratio of 0.71 indicates multistate melting for this compound. The sequence dependence of the thermodynamic quantities becomes apparent when the parameters of the alternating oligo(2'-deoxyxylonucleotide) d[(xA-xT)6-T] are compared to those of d[(xA)3-(xT)3-(xA)3-(xT)3-T). The values are delta Hcal = 330 +/- 30 kJ.mol-1; delta HvH = 180 +/- 15 kJ.(mol of cooperative unit)-1 delta HvH/ delta Hcal = 0.55. The transition enthalpy of the alternating oligo(2'-deoxyxylonucleotide) (B) is the highest but the cooperativity of transition is the lowest of the oligonucleotides studied. The circular dichroic spectra of the two oligo(2'-deoxyxylonucleotides) show unusual features in that d[(xA)3-(xT)3-(XA)3-(xT)3-T] exhibits a spectrum that is suggestive of a left-handed double helix, while the spectrum of the alternating oligo(2'-deoxyxylonucleotide) (B) resembles neither that of (C) nor that of (A).
三链 DNA 三路连接复合物形成的热力学。
影响因子：
2.9
作者：
Ladbury,JE;Sturtevant,JM;Leontis,NB
通讯作者：
Leontis,NB
糖基化在兔睾丸血管紧张素转换酶的生物合成和活性中的作用。
影响因子：
2.9
作者：
Kasturi,S;Jabbar,MA;Sen,GC;Sen,I
通讯作者：
Sen,I
DOI：
10.1073/pnas.83.11.3746
发表时间：
1986-06-01
影响因子：
11.1
作者：
BRESLAUER, KJ;FRANK, R;MARKY, LA
通讯作者：
MARKY, LA