Treatment of Acute Posterior Uveitis in a Porcine Model by Injection of Triamcinolone Acetonide Into the Suprachoroidal Space Using Microneedles
Treatment of Acute Posterior Uveitis in a Porcine Model by Injection of Triamcinolone Acetonide Into the Suprachoroidal Space Using Microneedles
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DOI：
10.1167/iovs.13-11747
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发表时间：
2013-04-01
影响因子：
4.4
通讯作者：
Patel, Samirkumar
中科院分区：
文献类型：
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作者：
Gilger, Brian C.;Abarca, Eva M.;Patel, Samirkumar
目的.在急性后段炎症猪模型中，评价使用微针将曲安奈德（TA）注入脉络膜上腔（SCS）的效果，并将其与玻璃体内（IVT）TA注射进行比较。IVT注射平衡盐溶液（BSS）或脂多糖（LPS）24小时后，向SCS或IVT注射0.2 mg或2.0 mg TA。在微创手术中使用微针进入SCS。每天收集眼部炎症评分和IOP测量值，而视网膜电图、光学相干断层扫描和宽视野眼底照相在治疗后-1，0和3天进行。还比较了房水和玻璃体液细胞计数和蛋白水平以及组织病理学。使用微针将TA递送至SCS简单、有效，并且与不良反应或毒性无关。SCS注射低剂量（0.2 mg）和高剂量（2.0 mg）TA在减少眼后段急性炎症方面与高剂量IVT注射一样有效。低剂量SCS TA在减轻炎症方面也有效;然而，低剂量IVT TA无效。本研究的结果表明，在急性后节炎症模型中，0.2 mg和2.0 mg SCS TA在减轻炎症方面与2.0 mg IVT TA注射同样有效。在猪眼SCS中注射TA后，未出现不良反应、IOP升高或手术或药物毒性证据。
PURPOSE. To evaluate the effect of triamcinolone acetonide (TA) administered into the suprachoroidal space (SCS) using a microneedle and compare it with intravitreal (IVT) TA injections in a porcine model of acute posterior segment inflammation.MATERIALS. An IVT injection of balanced salt solution (BSS) or lipopolysaccharide (LPS) was followed 24 hours later with an injection of 0.2 mg or 2.0 mg of TA into the SCS or IVT. The SCS was accessed using microneedles in a minimally invasive procedure. Ocular inflammatory scores and IOP measurements were collected daily, whereas electroretinography, optical coherence tomography, and wide-field ocular fundus photography was performed on -1, 0, and 3 days after treatment. Aqueous and vitreous humor cell counts and protein levels and histopathology were also compared.RESULTS. Delivery of TA to the SCS using microneedles was simple, effective, and not associated with adverse effects or toxicity. SCS injection of low (0.2 mg) and high doses (2.0 mg) of TA was as effective in reducing acute inflammation in the ocular posterior segment as high-dose IVT injection. Low-dose SCS TA was also effective in reducing inflammation; however, low-dose IVT TA was not.CONCLUSIONS. Results from this study suggest that 0.2 mg and 2.0 mg of SCS TA was as effective in reducing inflammation as 2.0 mg IVT TA injection in a model of acute posterior segment inflammation. There were no adverse effects, increased IOP, or evidence of procedural or drug toxicity following injection of TA into the SCS in porcine eyes.