PERMANENT ATTACHMENT OF REPLICATION ORIGINS TO THE NUCLEAR MATRIX IN BHK-CELLS
PERMANENT ATTACHMENT OF REPLICATION ORIGINS TO THE NUCLEAR MATRIX IN BHK-CELLS
复制标题
DOI：
10.1093/nar/14.8.3241
复制
发表时间：
1986-04-25
影响因子：
14.9
通讯作者：
PODDIGHE, J
中科院分区：
文献类型：
--
作者：
DIJKWEL, PA;WENINK, PW;PODDIGHE, J
通过放射自显影术分析复制起点和复制叉相对于核基质的位置。分析2 M氯化钠提取的BHK-核，准备覆盖嘴唇，表明短暂的脉冲后，颗粒被发现完全超过中央核心的残留核，这对应于在核基质中的位置。在连续的细胞中，这些颗粒被发现在随后的追踪过程中迁移到围绕基质的DNA晕中。然而，当在S期开始时对同步细胞施加脉冲时，观察到在大多数结构中没有发生这种迁移。从这一点，并从事实上，标签纳入后，在S-阶段可以追逐到晕，我们得出结论，相反的DNA复制叉，DNA含有复制起点是永久附着在核基质。
The position of replication origins and replication forks relative to the nuclear matrix was analysed by autoradiography. Analysis of 2M NaCl-extracted BHK-nuclei, prepared on covers lips, showed that after brief pulses grains were exclusively found over the central core of the residual nuclei, which corresponds to positions in the nuclear matrix. In asynchronuous cells these grains were found to migrate into the DNA-halo surrounding the matrix during a subsequent chase. When the pulse had been administered to synchro-nuous cells at the onset of S-phase, it was observed, however, that in the majority of the structures no such migration had occurred. From this, and from the fact that label incorporated later in S-phase could be chased into the halo, we conclude that, contrary to DNA in replication forks, DNA containing replication origins is permanently attached to the nuclear matrix.