Chiral discrimination using an immunosensor
Chiral discrimination using an immunosensor
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DOI：
10.1038/7927
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发表时间：
1999-04-01
影响因子：
46.9
通讯作者：
Green, BS
中科院分区：
文献类型：
--
作者：
Hofstetter, O;Hofstetter, H;Green, BS
基于免疫球蛋白的立体选择性，我们开发了一种新的手性传感器，用于检测低分子量分析物。使用表面等离子体共振检测，游离的未衍生化的α-氨基酸的对映异构体可以在竞争性测定中通过它们与这类物质的手性中心特异性抗体的相互作用来监测。对非外消旋混合物中的次要对映体的敏感性超过目前可用的方法;因此，这种免疫传感器可以容易地检测痕量的对映体杂质，并且对于科学和工业中的一系列应用是有吸引力的。
Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatized alpha-amino acids can be monitored in a competitive assay by their interaction with antibodies specific for the chiral center of this class of substances. The sensitivity to the minor enantiomer in nonracemic mixtures exceeds currently available methods; therefore, such immunosensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.