Microfluidic Synthesis of Barcode Particles for Multiplex Assays
Microfluidic Synthesis of Barcode Particles for Multiplex Assays
复制标题
用于多重检测的条码颗粒的微流控合成
DOI：
10.1002/smll.201401600
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发表时间：
2015
期刊：
影响因子：
13.3
通讯作者：
Gu Zhongze
中科院分区：
文献类型：
--
作者：
Zhao Yuanjin;Cheng Yao;Shang Luoran;Wang Jie;Xie Zhuoying;Gu Zhongze
在生物医学应用中，包括药物发现和临床诊断，越来越多地使用高通量检测，需要有效的多路复用策略。一种有前途的策略是使用条形码颗粒，其编码有关其特定成分的信息并能够进行简单的识别。已经提出了各种编码机制，包括光谱、图形、电子和物理编码，用于为条形码颗粒提供足够的识别码。这些粒子以各种方式合成。微流体技术是一种有效的方法，在条形码颗粒合成方面创造了令人兴奋的科学研究途径。所得颗粒在多种生物物种的检测中具有重要的应用，因为它们具有高灵活性、快速反应时间、较少试剂消耗和良好重复性的特性。本文综述了微流控合成用于多重检测的条形码颗粒的研究进展。在介绍了条形码粒子的发展策略后，重点介绍了微流体技术的研究，包括微流体技术的设计、制备及其在条形码粒子制备中的应用。将描述和强调所实现的条形码颗粒在多重测定中的应用。最后对这些条形码颗粒的未来发展进行了展望。
The increasing use of high‐throughput assays in biomedical applications, including drug discovery and clinical diagnostics, demands effective strategies for multiplexing. One promising strategy is the use of barcode particles that encode information about their specific compositions and enable simple identification. Various encoding mechanisms, including spectroscopic, graphical, electronic, and physical encoding, have been proposed for the provision of sufficient identification codes for the barcode particles. These particles are synthesized in various ways. Microfluidics is an effective approach that has created exciting avenues of scientific research in barcode particle synthesis. The resultant particles have found important application in the detection of multiple biological species as they have properties of high flexibility, fast reaction times, less reagent consumption, and good repeatability. In this paper, research progress in the microfluidic synthesis of barcode particles for multiplex assays is discussed. After introducing the general developing strategies of the barcode particles, the focus is on studies of microfluidics, including their design, fabrication, and application in the generation of barcode particles. Applications of the achieved barcode particles in multiplex assays will be described and emphasized. The prospects for future development of these barcode particles are also presented.