Effects of fetal exposure to urban particulate matter on the immune system of male mouse offspring.
Effects of fetal exposure to urban particulate matter on the immune system of male mouse offspring.
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DOI：
10.1248/bpb.b110708
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发表时间：
2012-08
影响因子：
2
通讯作者：
S. Yoshida;H. Takano;M. Nishikawa;H. Miao;T. Ichinose
中科院分区：
文献类型：
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作者：
S. Yoshida;H. Takano;M. Nishikawa;H. Miao;T. Ichinose
城市颗粒物（UPM）已被证明对成年小鼠的Th 2相关免疫系统有加重作用。然而，胎儿暴露于UPM对后代免疫应答的影响尚未阐明。在本研究中，我们在妊娠第7天和第14天通过阴道内注射给予妊娠母鼠UPM（200 µg/动物）。随后，9周龄和24周龄的雄性后代经气管内注射卵清蛋白（OVA）（4次，间隔2周），以建立支气管哮喘小鼠模型。然后，我们通过组织学检查结果，支气管肺泡灌洗液（BALF）中的炎性细胞数量，以及出生后5，10，15和30周BALF中细胞因子和趋化因子的蛋白浓度来评估后代过敏表现的进展。组织学检查表明，胎儿暴露于UPM单独引起轻微的嗜酸性粒细胞和淋巴细胞浸润的粘膜下层的气道和支气管上皮细胞和巨噬细胞的数量显着增加。此外，出生后卵母细胞内管理暴露于UPM在子宫内的后代引起的巨噬细胞，嗜酸性粒细胞和淋巴细胞的数量显着增加，并在其相关的细胞因子和趋化因子的浓度，表明胎儿暴露于UPM加重了化学致敏的免疫系统的男性后代。
Urban particulate matter (UPM) has been shown to have an aggravating effect on Th2-associated immune systems in adult mice. However, the effects of fetal exposure to UPM on immune response in offspring have not been elucidated. In the present study, we administered UPM (200 µg/animal) by intratracheal injection to pregnant dams on days 7 and 14 of gestation. Subsequently, 9- and 24-week-old male offspring were intratracheally injected with ovalbumin (OVA) (four times at 2-week intervals) to create a mouse model of bronchial asthma. We then evaluated the progression of allergic manifestations in the offspring through histological findings, the number of inflammatory cells in bronchoalveolar lavage fluid (BALF), and protein concentration of cytokines and chemokines in BALF 5, 10, 15, and 30 weeks after birth. Histological examination showed that fetal exposure to UPM alone caused slight eosinophil and lymphocyte infiltration in the submucosa of the airway and bronchial epithelium and significant increases in the number of macrophages. Moreover, postnatal intratracheal administration of OVA to offspring exposed to UPM in utero caused significant increases in the numbers of macrophages, eosinophils, and lymphocytes and in the concentrations of their relevant cytokines and chemokines, showing that fetal exposure to UPM aggravated the chemically sensitized immune system of male offspring.