Modulation of osteoblast function by prostaglandins.
Modulation of osteoblast function by prostaglandins.
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前列腺素调节成骨细胞功能。
DOI：
10.1152/ajprenal.1989.257.5.f755
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Muallem,S
中科院分区：
文献类型：
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作者：
Yamaguchi,DT;Green,J;Merritt,BS;Kleeman,CR;Muallem,S
研究了天然前列腺素(PGs)对成骨细胞系UMR-106-01和原代培养的新生大鼠颅骨成骨细胞内游离钙离子浓度([Ca+]i)、环磷酸腺苷(CAMP)水平和细胞增殖的影响。所有受试PG均能刺激细胞内[Ca~(2+)]_i升高，这主要是由于细胞内的钙离子释放所致。对不同PG的50%有效浓度的测量表明，PG诱导的细胞内[Ca~(2+)]_i升高的效力顺序为：F2α大于D2，远大于E_2，大于T_xB_2，大于E_1，大于I_2，远大于A_2。PGs还能增加成骨细胞中cAMP的水平。在所测试的最高浓度(10-25微米)下，只有PGE2和PGE1观察到cAMP产生的剂量-反应饱和。PG刺激cAMP升高的效价顺序为：E2大于E1，远大于A2，大于I2，大于F2，α大于D2，大于TxB2。PGs对胸腺嘧啶核苷摄取的影响的测量表明，低浓度的PGF2α和PGD2对成骨样细胞的增殖没有影响或促进了增殖。较低浓度的前列腺素E_2、前列腺素E_1和前列腺素A_2抑制细胞增殖。PG介导的抑制细胞增殖的效力等级与PG刺激的cAMP产生的效力等级相同。我们的结论是，所有自然产生的PG都能激活成骨细胞中的这两个信号转导系统。
The naturally occurring prostaglandins (PGs) were studied with respect to their abilities to change free cytosolic Ca2+ concentrations ([Ca2+]i), adenosine 3',5'-cyclic monophosphate (cAMP) levels, and cell proliferation in the osteoblastic cell line, UMR-106-01, and primary cultures of osteoblasts prepared from neonatal rat calvariae. All PGs tested stimulated an increase in [Ca2+]i, which was mainly due to Ca2+ release from intracellular stores. Measurements of the 50% effective concentration for the different PGs show that the potency ranking for PG-evoked [Ca2+]i increase in these cells is F2 alpha greater than D2 much greater than E2 greater than TxB2 greater than E1 greater than I2 much greater than A2. The PGs also increase cAMP levels in osteoblasts. At the highest concentrations tested (10-25 microM), dose-response saturation of cAMP production was observed only by PGE2 and PGE1. The potency rank for PG-stimulated cAMP increase was E2 greater than E1 much greater than A2 greater than I2 greater than F2 alpha greater than D2 greater than TxB2. Measurements of the effect of the PGs on thymidine uptake showed that low concentrations of PGF2 alpha and PGD2 had either no effect or stimulated proliferation of osteoblast-like cells. Relatively low concentration of PGE2, PGE1, and PGA2 inhibited proliferation. The potency ranking for PG-mediated inhibition of cell proliferation was identical to that found for PG-stimulated cAMP production. We conclude that all the naturally occurring PGs tested can activate the two signal transduction systems in osteoblasts.(ABSTRACT TRUNCATED AT 250 WORDS)