Nuclear transfer of perchloric acid-soluble protein by endoplasmic reticulum stressors
Nuclear transfer of perchloric acid-soluble protein by endoplasmic reticulum stressors
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DOI：
10.1110/ps.051481105
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发表时间：
2005-09-01
期刊：
影响因子：
8
通讯作者：
Minatogawa, Y
中科院分区：
文献类型：
--
作者：
Kanouchi, H;Matsumoto, M;Minatogawa, Y
高氯酸可溶性蛋白(PSP)在从细菌到哺乳动物的进化过程中高度保守。虽然PSP已被认为是体外翻译和增殖的抑制因子，但其确切的生物学作用尚未阐明。由于我们以前发现PSP与内质网(ER)和高尔基复合体的分布相似，所以我们对PSP在细胞内的分布进行了更详细的分析。免疫荧光研究表明，PSP与内质网和高尔基复合体共定位，因为PSP的分布模式与这两个细胞器都很匹配。免疫电子显微镜研究表明，PSP不仅位于胞浆中，而且位于内质网外膜的表面。由于PSP存在于内质网，我们推测它可能与内质网功能有关。因此，我们分析了作为ER功能之一的内质网应激反应是否影响了PSP的表达。结果表明，不同的内质网应激源(thapsigargin、A23187、衣霉素、布雷菲尔丁A和顺铂)在3h内引起PSP由核外到核内的定位发生显著变化，并且ER应激源诱导PSP的表达。这些结果表明，PSP参与了细胞对内质网应激源的反应，PSP从内质网到胞核的定位变化可能与内质网应激反应有关。
Perchloric acid-soluble protein (PSP) is highly conserved during evolution from bacteria to mammals. Although PSP has been recognized as an inhibitor of translation and proliferation in vitro, its precise biological role has not yet been elucidated. Since we previously found similar distributions for PSP and the endoplasmic reticulum (ER) and Golgi complex, the intracellular distribution of PSP was analyzed in more detail. Immunofluorescence studies indicated that PSP co-localized with the ER and Golgi complex, since the distribution pattern of PSP was well matched to both of these organelles. An immuncelectron microscopic study revealed PSP was located not only in the cytosol but also on the surface of the outer ER membrane. Since PSP was present on the ER, we speculated that it may be associated with ER function. Therefore, we analyzed whether or not the ER stress response, which is one of the ER functions, affected PSP expression. The results showed that various ER stressors (thapsigargin, A23187, tunicamycin, brefeldin A, and cisplatin) provoked a dramatic change in the localization of PSP from outside of the nucleus to inside the nucleus within 3 h. Moreover, the ER stressors induced PSP expression. These results suggest that PSP is involved in the cellular response to ER stressors, and that the change in localization of PSP from the ER to the nucleus may be associated with ER stress responses.