Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana
Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana
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DOI：
10.1046/j.1365-313x.1997.00527.x
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发表时间：
1997-09-01
期刊：
影响因子：
7.2
通讯作者：
Nam, HG
中科院分区：
文献类型：
--
作者：
Oh, SA;Park, JH;Nam, HG
从拟南芥（Arabidopsis thaliana）中分离出4个表现出延迟叶片衰老表型的突变体。遗传分析表明，它们都是单基因隐性突变，并落入三个互补组，确定了三个遗传位点控制叶片衰老拟南芥。在这些位点的突变导致延迟在所有衰老参数检查，包括叶绿素含量，光系统的光化学效率的tl，大亚基的Rubisco的相对量，核糖核酸酶和过氧化物酶的活性。在所有突变体植株中，在年龄依赖性的植株衰老和黑暗诱导的人工衰老过程中都观察到衰老症状的延迟。结果表明，突变所定义的3个基因是控制功能性叶片衰老的关键遗传元件，为拟南芥叶片衰老是由多个单基因位点控制的遗传程序化现象提供了决定性的遗传证据。结果进一步表明，这三个基因的功能在一个共同的步骤，年龄依赖性和黑暗诱导的衰老过程。进一步证明其中一个突变与乙烯不敏感突变ein 2 -1等位，证实了乙烯信号转导途径在拟南芥叶片衰老中的作用。
Four mutants that show the delayed leaf senescence phenotype were isolated from Arabidopsis thaliana. Genetic analyses revealed that they are all monogenic recessive mutations and fall into three complementation groups, identifying three genetic loci controlling leaf senescence iii Arabidopsis. Mutations in these loci cause delay in all senescence parameters examined, including chlorophyll content, photochemical efficiency of photosystem tl, relative amount of the large subunit of Rubisco, and RNase and peroxidase activity. Delay of the senescence symptoms was observed during both age-dependent in planta senescence and dark-induced artificial senescence in all of the mutant plants. The results indicate that the three genes defined by the mutations are key genetic elements controlling functional leaf senescence and provide decisive genetic evidence that leaf senescence is a genetically programmed phenomenon controlled by several monogenic loci in Arabidopsis. The results further suggest that the three genes function at a common step of age-dependent and dark-induced senescence processes. It is further shown that one of the mutations is allelic to ein2-1, an ethylene-insensitive mutation, confirming the role of ethylene signal transduction pathway in leaf senescence of Arabidopsis.