Molecular cloning of a new member of TEKTIN family, Tektin4, located to the flagella of rat spermatozoa
Molecular cloning of a new member of TEKTIN family, Tektin4, located to the flagella of rat spermatozoa
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DOI：
10.1002/mrd.20331
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发表时间：
2005-09-01
影响因子：
2.5
通讯作者：
Iida, H
中科院分区：
文献类型：
--
作者：
Matsuyama, T;Honda, Y;Iida, H
Tektins由与纤毛和鞭毛微管相关的致敏形成蛋白家族组成。通过PCR技术结合酵母双杂交筛选，获得了大鼠TEKTIN基因家族的一个新成员，命名为Tektin 4。大鼠Tektin 4 cDNA由1，341 bp组成，编码447个氨基酸的52 kDa蛋白。Tektin 4蛋白含有一个Tektin结构域，该结构域包含一个九肽签名序列（RPNVELCRD），这是Tektins的一个突出特征。其氨基酸序列与公共数据库中已登录的Tektin家族蛋白的同源性为29%~ 58%。Tektin 4基因定位于大鼠染色体10 q12，由6个外显子组成，全长5 kb。RT-PCR分析表明，Tektin 4主要在睾丸中表达，并在睾丸发育过程中表达上调。原位杂交结果显示，Tektin 4 mRNA定位于大鼠睾丸曲细精管圆形精子细胞中。Tektin 4蛋白主要定位于精子的鞭毛中，这表明它可能是鞭毛稳定性或精子运动所必需的鞭毛组分。(c)2005 Wiley-Liss，Inc.
Tektins are composed of a family of filament-forming proteins associated with ciliary and flagellar microtubules. A new member of the TEKTIN gene family, which was designated as rat Tektin4, was obtained by PCR technique combined with yeast two-hybrid screening. Rat Tektin4 cDNA consists of 1,341 bp encoding a 52 kDa protein of 447 amino acids. Tektin4 protein contains a Tektin domain including a nonapeptide signature sequence (RPNVELCRD), which is a prominent feature of Tektins. Its amino acid sequence showed 29%similar to 58% identities to that of other Tektin family proteins registered in the public databases. Tektin4 gene, which was mapped to rat chromosome 10q12, is composed of six exons and spanning 5 kb. Reverse-transcriptional-PCR (RT-PCR) analysis indicated that Tektin4 was predominantly expressed in testis and its expression was upregulated during testis development. In situ hybridization analysis showed that Tektin4 mRNA was localized in round spermatids in the seminiferous tubules of the rat testis. Tektin4 protein was predominantly localized in the flagella of spermatozoa, suggesting that it might works as a flagellar component requisite for flagellar stability or sperm motility. (c) 2005 Wiley-Liss, Inc.