Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2-mediated activation of the Nrf2/HO-1 pathway
Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2-mediated activation of the Nrf2/HO-1 pathway
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DOI：
10.1002/jcp.28347
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Giunta, Salvatore
中科院分区：
文献类型：
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作者：
Bucolo, Claudio;Drago, Filippo;Giunta, Salvatore
为了研究姜黄素对高糖（HG）刺激下人视网膜色素上皮（RPE）细胞的影响，我们对正常和高糖条件下培养的RPE细胞进行了体外研究，以评估姜黄素对细胞活力、核因子红细胞2相关因子2（Nrf 2）表达、HO-1活性和ERK 1/2表达的影响。暴露于HG损伤的RPE细胞用姜黄素处理。检测细胞活力、凋亡、HO-1活性、ERK和Nrf 2表达。数据表明用姜黄素处理引起细胞凋亡的显著减少。此外，姜黄素能够通过Nrf 2激活诱导HO-1表达，并抵消HG引起的损伤。目前的研究表明，姜黄素通过激活涉及ERK通路的Nrf 2/HO-1信号通路来保护RPE细胞免受HG诱导的损伤，这表明姜黄素可能在治疗糖尿病视网膜病变中具有治疗价值。
To study the effects of curcumin on human retinal pigment epithelial(RPE) cells exposed to high glucose (HG) insult, we performed in vitro studies on RPE cells cultured both in normal and HG conditions to assess the effects of curcumin on the cell viability, nuclear factor erythroid 2-related factor 2 (Nrf2) expression, HO-1 activity, and ERK1/2 expression. RPE cells exposed to HG insult were treated with curcumin. The cell viability, apoptosis, HO-1 activity, ERK, and Nrf2 expression were evaluated. The data indicated that treatment with curcumin caused a significant decrease in terms of apoptosis. Further, curcumin was able to induce HO-1 expression via Nrf2 activation and counteracts the damage elicited by HG. The present study demonstrated that curcumin provides protection against HG-induced damage in RPE cells through the activation of Nrf2/HO-1 signaling that involves the ERK pathway, suggesting that curcumin may have therapeutic value in the treatment of diabetic retinopathy.