Construction of mini-Tn5cyaA' and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica.
Construction of mini-Tn5cyaA' and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica.
复制标题
mini-Tn5cyaA的构建及其用于鉴定编码支气管败血博德特氏菌表面暴露和分泌蛋白的基因。
DOI：
10.1111/j.1574-6968.2001.tb10934.x
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发表时间：
2001
影响因子：
2.1
通讯作者：
Rosenshine,I
中科院分区：
文献类型：
--
作者：
Tu,X;Nisan,I;Miller,JF;Hanski,E;Rosenshine,I
A mini-Tn5transposon derivative, mini-Tn5cyaA′, has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain ofBordetella pertussisadenylate cyclase toxin (CyaA′). We used this system to mutagenizeB. bronchisepticaand we developed a screen for identification of mutants containingcyaA′ translational fusions. This system was used to identifyB. bronchisepticagenes that encode surface-exposed and secreted proteins.
