The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues.
The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues.
复制标题
骨脊椎动物中的SCPP基因曲目和矿化组织中的分级差异。
DOI：
10.1007/s00427-009-0276-x
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发表时间：
2009-03
影响因子：
2.4
通讯作者：
Kawasaki K
中科院分区：
文献类型：
--
作者：
Kawasaki K
脊椎动物的牙齿被牙釉质覆盖，大多数肉鳍动物的牙齿被牙釉质覆盖，而软骨鱼和放线鱼的牙齿则被牙釉质覆盖。这两种组织（身体中最硬的组织）与其他矿化组织之间的进化关系长期以来一直存在争议。我们最近报道了分泌性钙结合磷蛋白（SCPP）基因的特定组合与骨、牙本质、类牙釉质和牙釉质的矿化有关。因此，SCPP基因的早期曲目将阐明这些组织之间的进化关系。然而，SCPP基因在硬骨鱼和四足动物中的多样性以及这些基因在不同组织中的作用仍不清楚，主要是因为许多SCPP基因是谱系特异性的。在这项研究中，我展示了斑马鱼、青蛙和人类的SCPP基因库包括许多谱系特异性基因和一些起源于干骨鱼或更早的广泛保守的基因。表达分析表明，一些青蛙和斑马鱼的SCPP基因主要用于骨，但也用于牙本质，而其他基因则相反，类似于一些哺乳动物的SCPP基因。牙本质和类牙釉质最初在基质中使用共享基因，但类牙釉质随后过度矿化。值得注意的是，类珐琅质和珐琅质在超矿化过程中使用同源的SCPP基因。因此，牙釉质和牙釉质祖先的高矿化机制出现在放光翼类-肉翼类分化之前。然而，牙釉质使用特殊的SCPPs作为结构蛋白，而不是在搪瓷中使用，这反映了牙釉质与搪瓷质的差异。这些结果显示矿化牙组织的分级差异，并加强了骨-牙本质-牙釉质-牙釉质构成进化连续体的假设。
The vertebrate tooth is covered with enamel in most sarcopterygians or enameloid in chondrichthyans and actinopterygians. The evolutionary relationship among these two tissues, the hardest tissue in the body, and other mineralized tissues has long been controversial. We have recently reported that specific combinations of secretory calcium-binding phosphoprotein (SCPP) genes are involved in the mineralization of bone, dentin, enameloid, and enamel. Thus, the early repertoire of SCPP genes would elucidate the evolutionary relationship across these tissues. However, the diversity of SCPP genes in teleosts and tetrapods and the roles of these genes in distinct tissues have remained unclear, mainly because many SCPP genes are lineage-specific. In this study, I show that the repertoire of SCPP genes in the zebrafish, frog, and humans includes many lineage-specific genes and some widely conserved genes that originated in stem osteichthyans or earlier. Expression analysis demonstrates that some frog and zebrafish SCPP genes are used primarily in bone, but also in dentin, while the reverse is true of other genes, similar to some mammalian SCPP genes. Dentin and enameloid initially use shared genes in the matrix, but enameloid is subsequently hypermineralized. Notably, enameloid and enamel use an orthologous SCPP gene in the hyper-mineralization process. Thus, the hypermineralization machinery ancestral to both enameloid and enamel arose before the actinopterygian–sarcopterygian divergence. However, enamel employs specialized SCPPs as structuring proteins, not used in enameloid, reflecting the divergence of enamel from enameloid. These results show graded differences in mineralized dental tissues and reinforce the hypothesis that bone–dentin–enameloid–enamel constitutes an evolutionary continuum.
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DOI：
10.1073/pnas.0638023100
发表时间：
2003-04-01
影响因子：
11.1
作者：
Kawasaki, K;Weiss, KM
通讯作者：
Weiss, KM
影响因子：
4.1
作者：
Moffatt, Pierre;Smith, Charles E.;Nanci, Antonio
通讯作者：
Nanci, Antonio
影响因子：
10.7
作者：
Loh, Yong-Hwee;Brenner, Sydney;Venkatesh, Byrappa
通讯作者：
Venkatesh, Byrappa
DOI：
10.1007/s004290050185
发表时间：
1998-10-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
Huysseune, A;Van der Heyden, C;Sire, JY
通讯作者：
Sire, JY
DOI：
10.1073/pnas.0404279101
发表时间：
2004-08-03
影响因子：
11.1
作者：
Kawasaki, K;Suzuki, T;Weiss, KM
通讯作者：
Weiss, KM