7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers
7,8-Dihydroxyflavone nano-liposomes decorated by crosslinked and glycosylated lactoferrin: storage stability, antioxidant activity, in vitro release, gastrointestinal digestion and transport in Caco-2 cell monolayers
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DOI：
10.1016/j.jff.2019.103742
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Zhang, Ying
中科院分区：
文献类型：
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作者：
Chen, Yufeng;Xia, Guobin;Zhang, Ying
7,8-二羟黄酮（7,8- dhf）的低生物利用度和稳定性阻碍了其在预防人类疾病方面的潜在应用。制备了以原始的、交联的和糖基化的乳铁蛋白（LF）修饰的负载7,8- dhf的纳米脂质体。球形交联和糖基化LF包被脂质体的平均粒径比未包被脂质体大，多分散性指数较低，且包被效率较高（98%以上）。与游离7,8- dhf和负载7,8- dhf的脂质体相比，交联和糖基化的LF包覆脂质体具有更高的抗氧化活性、储存稳定性、缓释能力、生物可及性和经上皮转运能力。磷脂与7,8- dhf之间存在有效氢键，而LF蛋白与磷脂之间存在静电相互作用和氢键。通过差示扫描量热法检测，包封的7,8- dhf呈无定形而非晶体形态。总的来说，交联或糖基化的LF包被脂质体是保护和运输7,8- dhf或其他相关生物活性成分的有前途的递送系统。
Low bioavailability and stability of 7,8-dihydroxyflavone (7,8-DHF) hinder its potential application in prevention of humans' disease. 7,8-DHF loaded nanoliposomes decorated by original, crosslinked and glycosylated lactoferrin (LF) were fabricated. The mean particle size of spherical crosslinked and glycosylated LF coating liposomes were larger than uncoated liposomes with a low polydispersity index, and the former had higher encapsulation efficiency (Above 98%). Crosslinked and glycosylated LF coating liposomes had higher antioxidant activity, storage stability, slow-release ability, bioaccessibility and transepithelial transport compared to free 7,8-DHF and 7,8-DHF loaded liposome. Effective hydrogen bonding between phospholipid and 7,8-DHF, while electrostatic interaction and hydrogen bonding between LF proteins and phospholipid existed. The encapsulated 7,8-DHF was in an amorphous state rather than a crystalline form as detected by differential scanning calorimetry. Collectively, crosslinked or glycosylated LF coating liposomes are promising delivery systems for protecting and transporting 7,8-DHF or other relative bioactive components.