A thin-layer electrochemical detector coated with nafion film for liquid chromatography.
A thin-layer electrochemical detector coated with nafion film for liquid chromatography.
复制标题
一种涂有 nafion 薄膜的液相色谱薄层电化学检测器。
DOI：
10.1016/s0021-9673(00)90038-4
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
E. Wang
中科院分区：
文献类型：
--
作者：
H. Ji;E. Wang
研究了由涂有Nafion膜的玻碳电极组成的薄层电化学检测器的选择性和分析应用。由于Nafion聚合物的离子交换特性，大大提高了选择性和稳定性。涂层电极仅对阳离子具有良好的响应，其灵敏度与未涂层（裸）电极相同，但对阴离子不具有良好的响应，并且对中性分子的响应降低三倍。根据流动注射实验结果，讨论了电活性化合物在Nafion膜中的扩散。涂层电极上的峰电流与移动的流速无关。由于蛋白质吸附的电极中毒被最小化。在移动的相中使用30%甲醇或10%乙腈不影响涂覆电极的性能。具有三种电荷状态的各种分析物，即，测试阴离子、阳离子和中性。抗坏血酸，去甲肾上腺素，肾上腺素，多巴胺和尿酸的液相色谱电化学检测。
The selectivity and analytical application of a thin-layer electrochemical detector comprised of glassy carbon electrode coated with Nafion film were investigated. As a result of the ion-exchange characteristics of the Nafion polymer, the selectivity and stability were improved greatly. The coated electrode has a good response only for cations with the same sensitivity as an uncoated (bare) electrode, but not for anions, and the response for neutral molecules is decreased three-fold. The diffusion of electroactive compounds in Nafion film is discussed based on the results of flow injection experiments. The peak current at the coated electrode was independent of the flow-rate of the mobile phase. Electrode poisoning due to protein adsorption was minimized. The use of 30% methanol or 10% acetonitrile in the mobile phase did not affect the performance of the coated electrode. Various analytes having three kinds of charge state,i.e., anionic, cationic and neutral, were tested. Liquid chromatography with electrochemical detection of ascorbic acid, norepinephrine, epinephrine, dopamine and uric acid was demonstrated.