REQUIREMENT FOR DROSOPHILA CYTOPLASMIC TROPOMYOSIN IN OSKAR MESSENGER-RNA LOCALIZATION
REQUIREMENT FOR DROSOPHILA CYTOPLASMIC TROPOMYOSIN IN OSKAR MESSENGER-RNA LOCALIZATION
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DOI：
10.1038/377524a0
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发表时间：
1995-10-12
期刊：
影响因子：
64.8
通讯作者：
EPHRUSSI, A
中科院分区：
文献类型：
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作者：
ERDELYI, M;MICHON, AM;EPHRUSSI, A
oskar（osk）RNA定位于发育中的果蝇（Drosophila）卵母细胞的后极，诱导极浆的组装，导致腹部和生殖线的发育（1，2）。奥斯卡RNA定位失败导致胚胎缺乏腹部和生殖细胞。相反，oskar RNA错误靶向卵母细胞的前部会导致异位腹部和前极生殖细胞的形成（3）。极浆活性降低的母体突变体产生腹部发育正常的不育成虫，表明生殖细胞对极浆装配缺陷比腹部更敏感（4）。因此，降低oskar RNA定位或活性的基因突变可以恢复为可存活的不育成虫。在筛选生殖细胞形成缺陷突变体时，我们分离出原肌球蛋白II基因的9个等位基因（5）。在这里，我们表明，原肌球蛋白II（TmII）的突变几乎废除奥斯卡RNA定位到后极，这表明参与奥斯卡RNA定位的肌动蛋白网络。
THE localization of oskar (osk) RNA to the posterior pole of the developing fruit fly (Drosophila) oocyte induces the assembly of pole plasm, causing development of tbe abdomen and germ line(1,2). Failure to localize oskar RNA results in embryos that lack abdomen and germ cells. Conversely, mis-targeting of oskar RNA to the anterior of the oocyte causes formation of ectopic abdomen and germ cells at the anterior pole(3). Maternal mutants that have reduced pole plasm activity produce sterile adults with normal abdominal development, suggesting that germ cells are more sensitive than abdomen to defects in pole plasm assembly(4). Thus mutations in genes that reduce oskar RNA localization or activity can be recovered as viable sterile adults. In a screen for mutants defective in germ cell formation, we isolated nine alleles of the tropomyosin II gene(5). Here we show that mutations in tropomyosin II (TmII) virtually abolish oskar RNA localization to the posterior pole, suggesting an involvement of the actin network in oskar RNA localization.