Immunofluorescent study of alpha‐foetoprotein (αfp) in liver and liver tumours. I. Technique of αfp localization in tissue sections

Immunofluorescent study of alpha‐foetoprotein (αfp) in liver and liver tumours. I. Technique of αfp localization in tissue sections
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肝脏和肝脏肿瘤中甲胎蛋白 (αfp) 的免疫荧光研究 I. 组织切片中 αfp 定位的技术。

DOI:
10.1002/ijc.2910070203
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发表时间:
1971
影响因子:
6.4
通讯作者:
G. Abelev
G. Abelev
中科院分区:
医学1区
文献类型:
--
作者:
N. Engelhardt;A. I. Goussev;L. J. Shipova;G. Abelev

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用乙醇/乙酸固定的石蜡包埋组织块切片非常适合用于αfp的免疫荧光(IF)研究。抗原在石蜡块中保存至少1年。可以制备连续切片进行常规组织学检查,并在同一细胞中同时定位几种抗原。
Sections of paraffin‐embedded tissue blocks fixed by ethanol/acetic acid were found to be very suitable for immunofluorescent (IF) study of αfp. In paraffin blocks the antigen was preserved for at least 1 year. It was possible to prepare serial sections for routine histological examination and simultaneous IF localization of several antigens practically in the same cells.