GABAergic Complex Basket Formations in the Human Neocortex
GABAergic Complex Basket Formations in the Human Neocortex
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DOI：
10.1002/cne.22496
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发表时间：
2010-12-15
影响因子：
2.5
通讯作者：
DeFelipe, Javier
中科院分区：
文献类型：
--
作者：
Blazquez-Llorca, Lidia;Garcia-Marin, Virginia;DeFelipe, Javier
大脑皮层中的某些GABA能中间神经元与胞体建立多重连接，胞体通常勾勒出锥体细胞的体细胞和近端树突。在研究囊泡型GABA转运体(VGAT)在人脑皮质的分布时，我们发现在某些皮质区存在非常密集的细胞周围排列的多个VGAT免疫反应(-ir)终末。我们将这些终末称为“复杂的篮形结构”(CBK-结构)，以区别于大多数皮质神经元更简单和更典型的细胞周围GABA能神经支配。在这里，我们研究了这些VGAT-ir CBK结构在不同皮质区域的分布，包括躯体感觉(3b区)、视觉(17和18区)、运动(4区)、联合额叶(背外侧区9、10、45、46和眼眶11、12、13、14、47)、联合颞区(20、21、22和38区)和边缘扣带区(24、32区)。此外，我们使用双重或三重染色技术来研究神经支配细胞的化学性质。结果发现，VGAT-ir CBK阳性神经元主要分布在第4区，其次是3b区、13区和18区。此外，除了在IV层密度最高的17区外，VGAT-ir CBK阳性神经元主要分布在第III层。我们还发现，70%的支配神经元是锥体细胞，其余30%是多极细胞。这些多极细胞大多表达钙结合蛋白小白蛋白和凝集素Vicia villosa凝集素。J.Comp.神经。518：4917-4937,2010。(C)2010年Wiley-Liss公司
Certain GABAergic interneurons in the cerebral cortex, basket cells, establish multiple connections with cell bodies that typically outline the somata and proximal dendrites of pyramidal cells. During studies into the distribution of the vesicular GABA transporter (VGAT) in the human cerebral cortex, we were struck by the presence of a very dense, pericellular arrangement of multiple VGAT-immunoreactive (-ir) terminals in certain cortical areas. We called these terminals "Complex basket formations'' (Cbk-formations) to distinguish them from the simpler and more typical pericellular GABAergic innervations of most cortical neurons. Here we examined the distribution of these VGAT-ir Cbk-formations in various cortical areas, including the somatosensory (area 3b), visual (areas 17 and 18), motor (area 4), associative frontal (dorsolateral areas 9, 10, 45, 46, and orbital areas 11, 12, 13, 14, 47), associative temporal (areas 20, 21, 22, and 38), and limbic cingulate areas (areas 24, 32). Furthermore, we used dual or triple staining techniques to study the chemical nature of the innervated cells. We found that VGAT-ir Cbk-formations were most frequently found in area 4 followed by areas 3b, 13, and 18. In addition, they were mostly observed in layer III, except in area 17, where they were most dense in layer IV. We also found that 70% of the innervated neurons were pyramidal cells, while the remaining 30% were multipolar cells. Most of these multipolar cells expressed the calcium-binding protein parvalbumin and the lectin Vicia villosa agglutinin. J. Comp. Neurol. 518:4917-4937, 2010. (C) 2010 Wiley-Liss, Inc.