The Secretory Process and Yeast Cell-surface Assembly
The Secretory Process and Yeast Cell-surface Assembly
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分泌过程和酵母细胞表面组装
DOI：
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发表时间：
1982
期刊：
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通讯作者：
P. Novick
中科院分区：
文献类型：
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作者：
R. Schekman;P. Novick
引言本文的目的是回顾我们对酿酒酵母大分子分泌的了解，包括输出蛋白质和碳水化合物的合成和运动、参与该过程的膜细胞器，以及该过程的某些部分对细胞器相关蛋白定位的贡献。最近对本文中提出的一些材料进行了回顾（Cabib 1975；Farkas 1979；Byers 1981），并且 Ballou、Henry 和 Cooper 撰写的本卷中的章节是相关的。出芽生长的机制与此讨论相关。在大部分分裂周期中，细胞表面的生长仅限于芽。膜酶，例如葡聚糖合成酶，负责大部分这种局部生长，但质膜是连续的并包围着细胞的芽和母部分。因此，出芽生长意味着膜分化和表面成分定位的某种机制。表面生长和分泌之间的联系也引起了我们的注意。这些过程在出芽细胞中在时间和空间上同时发生。我们将提供支持分泌过程在芽形成中的作用的证据。尽管有风险，但借鉴其他分泌过程研究中得出的原理是很诱人的。本文包括一些类比和推测，我们希望这些类比和推测能够激发人们对酵母作为研究真核分泌和细胞表面生长的简单系统的兴趣。不幸的是，酵母并不是一种流行的原料......
INTRODUCTION The goal of this paper is to review our knowledge of macromolecular secretion in Saccharomyces cerevisiae in terms of the synthesis and movement of exported proteins and carbohydrates, the membrane organelles that participate in this process, and the contribution that parts of this process make to the localization of organelle-associated proteins. Some of the material presented in this paper has been reviewed recently (Cabib 1975; Farkas 1979; Byers 1981), and the chapters in this volume by Ballou, Henry, and Cooper are pertinent. The mechanism of budding growth is relevant to this discussion. During most of the division cycle, cell-surface growth is restricted to the bud. Membrane enzymes, such as glucan synthetase, are responsible for much of this localized growth, and yet the plasma membrane is continuous and surrounds both the bud and mother portions of the cell. Thus, budding growth implies some mechanism for membrane differentiation and localization of surface components. Our attention has also been drawn by the connection between surface growth and secretion. These processes coincide temporally and spatially in a budding cell. We will present the evidence that supports a role for the secretory process in the formation of a bud. It is tempting, though risky, to draw on principles derived from studies of other secretory processes. This paper includes some analogies and speculations that we hope will stimulate interest in yeast as a simple system for the study of eukaryotic secretion and cell-surface growth. Unfortunately, yeast has not been a popular source material for the...