Screening and Analyzing the Potential Bioactive Components from Reduning Injection, Using Macrophage Cell Extraction and Ultra-High Performance Liquid Chromatography Coupled with Mass Spectrometry
Screening and Analyzing the Potential Bioactive Components from Reduning Injection, Using Macrophage Cell Extraction and Ultra-High Performance Liquid Chromatography Coupled with Mass Spectrometry
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DOI：
10.1142/s0192415x1350016x
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发表时间：
2013-01-01
影响因子：
5.7
通讯作者：
Yu, Li
中科院分区：
文献类型：
--
作者：
Li, Yonghui;Wang, Peixiang;Yu, Li
本文采用巨噬细胞提取、超高效液相色谱-质谱联用技术，建立了热毒宁注射液中潜在活性成分的筛选和分析方法。此外，还研究了其对LPS诱导的巨噬细胞损伤的保护作用。结果表明，绿原酸、3，4-二咖啡酰奎宁酸、3，5-二咖啡酰奎宁酸和4，5-二咖啡酰奎宁酸均能显著抑制巨噬细胞分泌前列腺素E2（PGE 2），逆转巨噬细胞分泌白细胞介素6（IL 6）（p <0.05）。结果表明，巨噬细胞提取法与超高效液相色谱-质谱联用技术相结合，可用于中药注射液中潜在活性成分的筛选和分析。
In this paper, a useful method for screening and analyzing the potential bioactive components from Reduning Injection was developed using macrophage cell extraction and ultra-high performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on macrophage cell damage induced by LPS in vitro were also investigated. The results showed that chlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid significantly inhibited the prostaglandin E-2 (PGE(2)) release and reversed the interleukin-6 (IL-6) secretion of macrophages (p < 0: 05). These data indicated that the method of macrophage cell extraction coupled with UPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCM injection.