A novel 53 kDa protein (BoP53) in Babesia orientalis poses the immunoreactivity using the infection serum
A novel 53 kDa protein (BoP53) in Babesia orientalis poses the immunoreactivity using the infection serum
复制标题
东方巴贝虫中的一种新型 53 kDa 蛋白 (BoP53) 使用感染血清产生免疫反应性
DOI：
10.1016/j.parint.2020.102152
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发表时间：
2020-10-01
影响因子：
1.9
通讯作者：
Zhao, Junlong
中科院分区：
文献类型：
--
作者：
Shu, Xiang;Guo, Jiaying;Zhao, Junlong
东方巴氏木（Babeanorientalis，B. orientalis）是引起我国南方布法罗巴贝斯虫病的主要病原菌，在我国南方曾造成严重的经济损失。虽然入侵过程已被大致描述，但仍有一些未知分子尚未被识别。近年来，在牛Babybovis中发现了一种与侵袭相关的蛋白BOV 57。然而，在B中该基因的研究尚未见报道。东方B。用B中的Blast BOV 57序列获得东方梭菌P53（BoP 53）序列。orientalis基因组数据库中获得，BoP 53基因全长为1599 bp。将BoP 53基因克隆到pGEX-6P-1表达载体中，并以GST标签融合蛋白的形式表达。用I-TASSER软件预测了BoP 53的三级结构。从B中鉴定出天然BoP 53。用抗rBoP 53的小鼠抗血清孵育东方红细胞裂解物。BoP 53蛋白作为一种新的蛋白质，对B的研究起到了重要的推动作用。rBoP 53与B.东方蜱感染布法罗血清而非健康布法罗血清表明其具有良好的抗原性。有可能成为诊断B的候选抗原。东方感染
Babesia orientalis (B. orientalis) is responsible for water buffalo babesiosis, which caused serious economic losses in the south of China. Although the invasion process has been roughly described, there are still some unknown molecules that have not yet been identified. Recently, an invasion-related protein BOV57 has been identified in the Babesia bovis. However, there is no report available about the gene in B. orientalis. B. orientalis P53 (BoP53) sequence was obtained by blast BOV57 sequence in B. orientalis genome database, and the full length of the BoP53 gene is 1599 bp. BoP53 gene was cloned into a pGEX-6P-1 expression vector and expressed as a GST-tag fusion protein. The tertiary structure of BoP53 was predicted with the I-TASSER software. The native BoP53 was identified from of B. orientalis lysate incubation with mouse antiserum against rBoP53. BoP53 as a novel identified protein promotes the study of B. orientalis, the reaction of rBoP53 with the serum of B. orientalis-infected water buffalo but not with healthy buffalo serum indicated its good antigenicity. It may be a candidate antigen for the diagnosis of B. orientalis infection.