ANTIGENIC DOMAINS ON THE PEPLOMER PROTEIN OF AVIAN INFECTIOUS-BRONCHITIS VIRUS - CORRELATION WITH BIOLOGICAL FUNCTIONS
ANTIGENIC DOMAINS ON THE PEPLOMER PROTEIN OF AVIAN INFECTIOUS-BRONCHITIS VIRUS - CORRELATION WITH BIOLOGICAL FUNCTIONS
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DOI：
10.1099/0022-1317-71-9-1929
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发表时间：
1990-09-01
影响因子：
3.8
通讯作者：
VANROOZELAAR, DJ
中科院分区：
文献类型：
--
作者：
KOCH, G;HARTOG, L;VANROOZELAAR, DJ
制备了抗传染性支气管炎病毒（IBV）结构蛋白的单克隆抗体（MAbs），用于分析该病毒的抗原结构。在抗体结合试验中分析酶标记和未标记的MAb与IBV peplomer蛋白的竞争性结合，以测试MAb所定义的表位的相关性。基于竞争组，定义了8个表位簇（S-A至S-H）;这些簇中的6个（S1-A至S1-F）位于peplomer蛋白的S1亚基上，2个（S2-G和S2-H）位于S2亚基上。表位簇S1-A和S1-B广泛重叠。测定单克隆抗体的生物学活性并与表位簇相关。针对簇S2-G的单克隆抗体以高于2 log 10的滴度中度至强烈中和IBV，而针对S1和S2的剩余MAb以低于2 log 10的滴度中和。一个单克隆抗体，针对簇S1-D，抑制鸡红细胞凝集。
Monoclonal antibodies (MAbs) directed against structural proteins of infections bronchitis virus (IBV) were produced to analyse the antigenic structure of this virus. Competitive binding of enzyme-labelled and unlabelled MAbs to IBV peplomer protein was analysed in an antibody binding assay to test the relatedness of the epitopes defined by the MAbs. Based on the competition groups, eight epitope clusters were defined (S-A to S-H); six of these clusters (S1-A to S1-F) were located on the S1 subunit and two (S2-G and S2-H) on the S2 subunit of the peplomer protein. Epitope clusters S1-A and S1-B overlapped extensively. The biological activities of the MAbs were determined and correlated to the epitope clusters. Monoclonal antibodies directed against cluster S2-G moderately to strongly neutralized IBV at titres higher than 2 log10, whereas the remaining MAbs, directed against S1 and S2, neutralized at titres lower than 2 log10. One MAb, directed against cluster S1-D, inhibited the agglutination of chicken erythrocytes.