Development and comparison of RAPD, SCAR and AFLP markers for distinguishing some medicinally important species of the genus Phyllanthus
Development and comparison of RAPD, SCAR and AFLP markers for distinguishing some medicinally important species of the genus Phyllanthus
复制标题
DOI：
10.1080/11263504.2011.635714
复制
发表时间：
2013-03-01
期刊：
影响因子：
2
通讯作者：
Raychaudhuri, S. S.
中科院分区：
文献类型：
--
作者：
Bandyopadhyay, S.;Raychaudhuri, S. S.
随机扩增多态性脱氧核糖核酸（RAPD），扩增片段长度多态性（AFLP）和序列特征扩增区域（SCAR）标记，用于正确鉴定叶下珠属的5个药用重要物种，从而使这些物种被用于草药工业后，直接从外地。RAPD数据将草药P.urinaria与其余物种分离，而AFLP数据成功区分了所有草药，即，苦木，（P.fraternus韦伯斯特）和P.urinaria L.一个SCAR标记的草本物种，P.urinaria，也被开发。AFLP是一种较RAPD更好的多态性标记。
Random amplified polymorphic deoxyribonucleic acid (RAPD), amplified fragment length polymorphism (AFLP) and sequence characterized amplified region (SCAR) markers were employed for proper identification of five medicinally important species from the genus Phyllanthus, thus allowing these species to be used in herbal drug industry straight after bringing them from the field. RAPD data segregated the herb P. urinaria from the remaining species, whereas AFLP data successfully distinguished all the herbs, i.e., P. amarus Schum., P. fraternus Webster and P. urinaria L. A SCAR marker for one of the herbaceous species, P. urinaria, was also developed. In this study, AFLP came out as a better polymorphic marker than RAPD.