Gene expression and maintenance of pregnancy in bovine: Roles of trophoblastic binucleate cell-specific molecules
Gene expression and maintenance of pregnancy in bovine: Roles of trophoblastic binucleate cell-specific molecules
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DOI：
10.1071/rd06118
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发表时间：
2007-01-01
影响因子：
1.9
通讯作者：
Takahashi, Toru
中科院分区：
文献类型：
--
作者：
Hashizume, Kazuyoshi;Ushizawa, Koichi;Takahashi, Toru
细胞间的相互作用在胎盘发生的调控和胎儿与母体单位之间阶段特异性发育信号的交换中起着关键作用。具体而言，这些相互作用对于胎儿的程序性生长、母体对妊娠的适应和分娩的协调至关重要。然而，关于牛胎盘形成和妊娠维持的精确调节知之甚少。因此，本研究的目的是破译细胞通信的复杂网络，以深入了解多方面的发育过程，并了解有缺陷的通信的深刻后果。在反刍动物中，双核细胞在形成母胎界面的结构和分泌物方面起着核心作用，这些结构和分泌物对建立和维持妊娠至关重要。在此，我们总结了在牛子叶和肉阜中使用全球基因表达谱的特定RNA转录物丰度的差异，并进一步研究了选定的妊娠特异性感兴趣的基因（从微阵列研究中鉴定）的mRNA丰度的关系，这些基因仅局限于双核细胞，如胎盘催乳素，催乳素相关蛋白和妊娠相关糖蛋白。结果表明，一个精心策划的转录命令从双核细胞是关键的建立和发展的怀孕牛。
Cell to cell interaction plays a pivotal role in the regulation of placentogenesis and exchange of stage-specific developmental signals between the fetal and maternal units. Specifically, these interactions are paramount for programmed fetal growth, maternal adaptation to pregnancy and coordination of parturition. However, little is known about the precise regulation of placentation and maintenance of gestation in cattle. Therefore, the aim of the present study was to decipher the complex networks of cell communication to gain an insight into the multifaceted developmental process and understand the profound consequences of flawed communication. In the ruminant, the binucleate cell plays a central role in forming the structures and secretions at the fetomaternal interface that are crucial in establishing and maintaining pregnancy. Herein, we summarise differences in the abundance of specific RNA transcripts in the bovine cotyledon and caruncle using global gene expression profiling and further investigate the relationship of mRNA abundance for selected pregnancy-specific genes of interest (identified from microarray studies) that are localised exclusively to the binucleate cell, such as placental lactogen, prolactin-related proteins and pregnancy-associated glycoproteins. The results suggest that a well-orchestrated transcriptional command from binucleate cells is pivotal to the establishment and progression of pregnancy in cattle.