Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells
Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells
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DOI：
10.1016/j.biomaterials.2008.01.024
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发表时间：
2008-05-01
期刊：
影响因子：
14
通讯作者：
Ramakrishna, Seeram
中科院分区：
文献类型：
--
作者：
Ma, Kun;Chan, Casey K.;Ramakrishna, Seeram
骨髓源性造血干细胞 (BM-HSC) 与其局部微环境之间的相互作用是 BM-HSC 迁移、增殖和分化信号控制的重要组成部分。我们假设基质地形和生化信号都会促进 BM-HSC 的粘附行为，这对于 BM-HSC 在其微环境中的归巢、自我更新和谱系承诺至关重要。我们采用静电纺丝技术制造了聚（DL-丙交酯-乙交酯）与 I 型胶原蛋白混合的纳米纤维支架（NFS）。NFS 进一步涂覆有 E-选择素（一种关键的粘附生物分子）。捕获效率研究表明，混合 NFS 包被 E-选择素后，在室温孵育 30 分钟内，细胞捕获率从 23.40% 显着提高到 67.41%，在 60 分钟内从 29.44% 显着提高到 70.19%。这项研究强调了使用仿生支架设计特定位点的利基样单元以促进 BM-HSC 增殖或分化功能的潜力。 (c) 2008 Elsevier Ltd. 保留所有权利。
Interactions between bone marrow-derived hematopoietic stem cells (BM-HSCs) and their local microenvironment are an integral part of signaling control of BM-HSCs migration, proliferation and differentiation. We hypothesized that both substrate topographical and biochemical cues promote BM-HSCs adhesive behaviors, which are crucial for BM-HSCs' homing, self-renewal and lineage commitment within their microenvironment. We employed electrospinning technique to fabricate nanofiber scaffolds (NFS) with poly(DL-lactide-co-glycolide) blended with collagen I. NFS was further coated with E-selectin, a critical adhesive biomolecule. Capture efficiency study showed that blended NFS, after coated with E-selectin, significantly increased cell capture percentage from 23.40% to 67.41% within 30 min and from 29.44% to 70.19% within 60 min of incubation at room temperature. This study highlights the potential of using a biomimetic scaffold to design a site-specific niche-like unit for facilitating proliferation or differentiation functions of BM-HSCs. (c) 2008 Elsevier Ltd. All rights reserved.