TFP5 is comparable to mild hypothermia in improving neurological outcomes in early-stage ischemic stroke of adult rats
TFP5 is comparable to mild hypothermia in improving neurological outcomes in early-stage ischemic stroke of adult rats
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DOI：
10.1016/j.neuroscience.2016.12.009
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发表时间：
2017-02
期刊：
影响因子：
3.3
通讯作者：
Ya-bin Ji;Pei-Pei Zhuang-Pei;Z. Ji;Kaibin Huang;Yong Gu;Yongming Wu;S. Pan;Yafang Hu
中科院分区：
文献类型：
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作者：
Ya-bin Ji;Pei-Pei Zhuang-Pei;Z. Ji;Kaibin Huang;Yong Gu;Yongming Wu;S. Pan;Yafang Hu
目的：我们比较了修饰的截短的24-aa肽（TFP 5），来自细胞周期蛋白依赖性激酶5（CDK 5）激活辅因子p35，与轻度低温（MH）的疗效，并确定是否TFP 5的疗效是由MH.Methods：缺血性中风诱导成年雄性SD大鼠2小时。在开始再灌注后立即给予TFP 5、MH或两者的组合。再灌注48 h后，对神经功能进行评估。结果：与常温对照组（NT）相比，接受MH、TFP 5或联合治疗的大鼠脑梗死面积较小，且三个治疗组之间无明显差异。神经功能缺损仅通过联合治疗得到显著改善。MH或TFP 5改善缺血区血脑屏障（BBB）破坏的效果相似，而两者联合使用有更好的效果趋势。此外，裂解的p35到p25和缺血区域的细胞凋亡被TFP 5或组合抑制，但不是由MH单独。结论：TFP 5是可比MH在改善早期成人缺血性中风的神经功能的结果。当TFP 5与MH一起沿着给予时，倾向于实现较少的神经功能缺损。
Aim: We compared the efficacy of a modified truncated 24-aa peptide (TFP5), derived from the cyclin-dependent kinase 5 (CDK5)-activating cofactor p35, with mild hypothermia (MH), and determined whether the efficacy of TFP5 is affected by MH.Methods: Ischemic stroke was induced in adult male Sprague–Dawley rats for 2 h. Immediately after initiating reperfusion, TFP5, MH, or the combination of the two were administrated. 48 h after reperfusion, neurological outcomes were evaluated.Results: Rats that received either MH, TFP5, or the combined treatment showed smaller brain infarct size than normothermia control (NT), and there was no apparent difference among these three treatment groups. The neurological deficit was significantly improved only by the combined treatment. MH or TFP5 ameliorated the blood–brain barrier (BBB) disruption in ischemic regions with similar efficacy, whereas the combination of them had a trend toward better effect. Besides, the cleavage of p35 into p25 and apoptosis in ischemic regions was inhibited by TFP5 or the combination, but not by MH alone.Conclusions: TFP5 is comparable to MH in improving neurological outcomes in early-stage adult ischemic stroke. When TFP5 is given along with MH, less neurological deficit tends to be achieved.