Angiotensin I converting enzyme from human plasma.
Angiotensin I converting enzyme from human plasma.
复制标题
人血浆中的血管紧张素 I 转换酶。
DOI：
10.1021/bi00644a015
复制
发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
B. Fanburg
中科院分区：
文献类型：
--
作者：
J. Lanzillo;B. Fanburg
血管紧张素I转化酶纯化101 000倍，从人血浆中的同质性，通过色谱和电泳技术的组合。这种酶类似于其他血管紧张素I转换酶。它是一种酸性糖蛋白，由分子量为140 000的单一多肽链组成，等电点为4.6。该酶需要氯离子才能发挥活性，并被乙二胺四乙酸、血管紧张素II、缓激肽、缓激肽增强因子九肽和3-巯基-2-D-甲基丙酰基-L-脯氨酸（SQ-14，225）抑制。纯化的制剂裂解缓激肽以及血管紧张素II和马尿酰-L-组氨酰-L-亮氨酸。其与血管紧张素I的比活性为2.4单位/mg，与马尿酰-L-组氨酰-L-亮氨酸的比活性为31.4单位/mg。
The angiotensin I converting enzyme was purified 101 000-fold to homogeneity from human plasma by a combination of chromatographic and electrophoretic techniques. The enzyme is similar to other angiotensin I converting enzymes. It is an acidic glycoprotein consisting of a single polypeptide chain of molecular weight 140 000 with an isoelectric point of 4.6. The enzyme requires chloride ion for activity and is inhibited by ethylenediaminetetraacetic acid, angiotensin II, bradykinin, bradykinin potentiating factor nonapeptide, and 3-mercapto-2-D-methylpropanoyl-L-proline (SQ-14,225). The purified preparation cleaves bradykinin as well as angiotensin II and hippuryl-L-histidyl-L-leucine. Its specific activity with angiotensin I is 2.4 units per mg and with hippuryl-L-histidyl-L-leucine is 31.4 units per mg.