Microinjections of anisomycin into the intermediate cerebellum during learning affect the acquisition of classically conditioned responses in the rabbit
Microinjections of anisomycin into the intermediate cerebellum during learning affect the acquisition of classically conditioned responses in the rabbit
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DOI：
10.1016/s0006-8993(97)01535-7
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发表时间：
1998-03-30
期刊：
影响因子：
2.9
通讯作者：
Bloedel, JR
中科院分区：
文献类型：
--
作者：
Bracha, V;Irwin, KB;Bloedel, JR
本研究的目的是研究兔小脑中间区蛋白质合成抑制对经典条件反射的获得和表达的影响。在标准延迟范例中，动物被训练了三天。每次训练前，双侧小脑间隔核注射一种山奈霉素液(一种蛋白质合成抑制剂)或赋形剂。在这三次训练后，对兔子进行测试，以确定先前在茴香素或赋形剂影响下的训练是否导致条件反应的获得。在这项测试中，之前注射蛋白质合成抑制剂的动物比注射了交通工具的兔子对条件反应的保留要少得多。另外的实验表明，在条件化过程中或在训练有素的动物身上，茴香素不会阻止条件性反应的表达。在之前药物注射的相同部位微量注射蝇草酚抑制了条件性反应的表达，表明蛋白质合成抑制剂应用于小脑环路的眨眼相关部分。获得的数据首次证明，对小脑回路的操纵不会影响学习行为的表现，可以影响学习过程。这些结果表明，中间小脑中新蛋白质的合成参与了导致眨眼条件反射的可塑性变化的形成。(C)1998年爱思唯尔科学公司。
The purpose of this study was to examine the effects of protein synthesis inhibition in the intermediate cerebellum on the acquisition and expression of classically conditioned nictitating membrane responses in the rabbit. Animals were conditioned for three days in a standard delay paradigm. Before each training session, either a solution of anisomycin (a protein synthesis inhibitor) or vehicle was bilaterally injected into the interposed cerebellar nuclei. Following these three training sessions, rabbits were tested to determine whether the previous training under the influence of anisomycin or vehicle resulted in the acquisition of conditioned responses. In this test, animals that were injected previously with the protein synthesis inhibitor exhibited significantly less retention of conditioned responses than rabbits injected with vehicle. Additional experiments demonstrated that anisomycin does not block the expression of conditioned responses during conditioning or in well-trained animals. Microinjections of muscimol at the same sites of the previous drug infusions suppressed the expression of conditioned responses, indicating that the protein synthesis inhibitor was applied to the eyeblink-related parts of cerebellar circuits. The obtained data are the first to demonstrate that a manipulation of cerebellar circuits, which does not affect the performance of learned behavior, can affect the process of learning. These results suggest that the synthesis of new proteins in the intermediate cerebellum participates in the formation of plastic changes responsible for eyeblink conditioning. (C) 1998 Elsevier Science B.V.