ORGANELLE DYNAMICS IN LOBSTER AXONS - ANTEROGRADE, RETROGRADE AND STATIONARY MITOCHONDRIA
ORGANELLE DYNAMICS IN LOBSTER AXONS - ANTEROGRADE, RETROGRADE AND STATIONARY MITOCHONDRIA
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DOI：
10.1016/0006-8993(87)91443-0
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发表时间：
1987-05-26
期刊：
影响因子：
2.9
通讯作者：
SMITH, RS
中科院分区：
文献类型：
--
作者：
FORMAN, DS;LYNCH, KJ;SMITH, RS
用微分干涉对比光学和视频显微镜技术对龙虾足运动神经轴突的线粒体进行了观察。线粒体的运动在延时录像带记录中进行了分析。逆行方向的平均输运速度（1.33 ±. 0.64μ m/s）大于在顺行方向上的平均运输速度（0.72 ± 0.55 μ m/s）。0.26μ m/s）。沿逆行和顺行方向移动的线粒体的平均长度仅略有不同（分别为6.9 μ m和5.5 μ m）。线粒体长度与平均速度和倒数速度无相关性。线粒体的瞬时速度分布在约3 μ m/s的范围内;顺行和逆行分布都包含一小部分符号与模态值相反的值。瞬时速度的变化发生在接近0.1 Hz的频率。一些线粒体显示类似的低频纵向振荡运动。虽然大多数线粒体的运动是平行于轴的轴突，横向偏差和复杂的圆形路径有时被观察到。有些线粒体改变了方向，继续朝同一方向运动，因此，原来的前端变成了尾端。当轴浆结构被破坏时，质膜下的许多线粒体是静止的，并强烈地粘附在质膜上。在电子显微镜下，细链连接外周线粒体和质膜。这些链可以将静止的线粒体锚在质膜上。
Mitochondria in isolated motor axons from the walking legs of lobster were observed with differential interference contrast optics and video microscopic techniques. Movements of the mitochondria were analyzed in time-lapse videotape records. The mean velocity of transport in the retrograde direction (1.33 .+-. 0.64 .mu.m/s) was greater than the mean velocity of transport in the anterograde direction (0.72 .+-. 0.26 .mu.m/s). The mean lengths of the mitochondria moving in the retrograde and anterograde directions were only slightly different (6.9 .mu.m and 5.5 .mu.m, respectively). No correlation was found between mitochrondrial length and average velocity or reciprocal velocity. The instantaneous velocities of mitochondria were distributed over a range of approximately 3 .mu.m/s; both the anterograde and retrograde distributions contained a small proportion of values whose sign was opposite to the modal value. The variation in instantaneous velocity took place at frequencies close to 0.1 Hz. Some mitochondria displayed longitudinally oriented oscillatory movements of a similar low frequency. While the movement of most mitochondria was parallel to the axis of the axon, transverse deviations and complex circular paths were sometimes observed. Some mitochondria reversed their orientation and continued in the same direction, so that the end which has been the leading end became the trailing end. Many mitochondria immediately beneath the plasma membrane were stationary and adhered strongly to the plasma membrane when the axoplasmic structure was disrupted. In electron micrographs, fine strands connected peripheral mitochondria and the plasma membrane. These strands may anchor the stationary mitochondria to the plasma membrane.