Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis.
Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis.
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DOI：
10.1093/dnares/dsn013
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发表时间：
2008-08
期刊：
影响因子：
--
通讯作者：
Nakayama K
中科院分区：
文献类型：
--
作者：
Naito M;Hirakawa H;Yamashita A;Ohara N;Shoji M;Yukitake H;Nakayama K;Toh H;Yoshimura F;Kuhara S;Hattori M;Hayashi T;Nakayama K
革兰氏阴性厌氧细菌牙龈卟啉单胞菌是慢性牙周炎的主要病原体。通过小鼠皮下软组织脓肿模型分析，将牙龈卟啉单胞菌菌株分为强毒株和弱毒株。在这里，我们展示了牙龈卟啉单胞菌 ATCC 33277 的全基因组序列，该菌株被归类为毒力较低的菌株。我们在 ATCC 33277 基因组中鉴定了 2090 个蛋白质编码序列 (CDS)、4 个 RNA 操纵子和 53 个 tRNA 基因。通过与强毒株 W83 进行基因组比较，我们鉴定了 461 个 ATCC 33277 特异性 CDS 和 415 个 W83 特异性 CDS。在这两个菌株之间观察到广泛的基因组重排：鉴定出 175 个发生基因组重排的区域。其中 35 次基因组重排是倒位或易位，140 次是简单的插入、删除或替换。两种菌株均含有大量可移动元件，例如插入序列、微型反向重复转座元件（MITE）和接合转座子，这些元件通常与基因组重排相关。这些发现表明，可移动遗传元件深深参与了牙龈卟啉单胞菌广泛的基因组重排和许多菌株特异性CDS的发生。我们还在这里描述了 MITE400 的一个非常独特的功能，我们将其重新命名为 MITEPgRS（具有重复序列的 P.gingivalis MITE）。
The gram-negative anaerobic bacterium Porphyromonas gingivalis is a major causative agent of chronic periodontitis. Porphyromonas gingivalis strains have been classified into virulent and less-virulent strains by mouse subcutaneous soft tissue abscess model analysis. Here, we present the whole genome sequence of P. gingivalis ATCC 33277, which is classified as a less-virulent strain. We identified 2090 protein-coding sequences (CDSs), 4 RNA operons, and 53 tRNA genes in the ATCC 33277 genome. By genomic comparison with the virulent strain W83, we identified 461 ATCC 33277-specific and 415 W83-specific CDSs. Extensive genomic rearrangements were observed between the two strains: 175 regions in which genomic rearrangements have occurred were identified. Thirty-five of those genomic rearrangements were inversion or translocation and 140 were simple insertion, deletion, or replacement. Both strains contained large numbers of mobile elements, such as insertion sequences, miniature inverted-repeat transposable elements (MITEs), and conjugative transposons, which are frequently associated with genomic rearrangements. These findings indicate that the mobile genetic elements have been deeply involved in the extensive genome rearrangement of P. gingivalis and the occurrence of many of the strain-specific CDSs. We also describe here a very unique feature of MITE400, which we renamed MITEPgRS (MITE of P. gingivalis with Repeating Sequences).
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影响因子：
3.1
作者：
Dong, H;Chen, TT;Duncan, MJ
通讯作者：
Duncan, MJ
影响因子：
7.6
作者：
Joshipura, KJ;Rimm, EB;Willett, WC
通讯作者：
Willett, WC
影响因子：
3.1
作者：
Aduse-Opoku, J;Slaney, JM;Curtis, MA
通讯作者：
Curtis, MA
影响因子：
3.1
作者：
Hall, LMC;Fawell, SC;Curtis, MA
通讯作者：
Curtis, MA
影响因子：
3.2
作者：
Bacic, M;Parker, AC;Smith, CJ
通讯作者：
Smith, CJ