Domain dynamics of phase-separated lipid membranes under shear flow
Domain dynamics of phase-separated lipid membranes under shear flow
复制标题
剪切流作用下相分离脂膜的畴动力学
DOI：
10.1039/d2sm00825d
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发表时间：
2022-11-24
期刊：
影响因子：
3.4
通讯作者：
Kitahata, Hiroyuki
中科院分区：
文献类型：
--
作者：
Hamada, Tsutomu;Mizuno, Shino;Kitahata, Hiroyuki
本文报道了相分离脂囊泡在外部流动下的侧区动力学行为。微流控室用于囊泡的固定和剪切的应用。微观观察发现，域往往是本地化的涡中心，并表现出条纹形态的流速增加。我们澄清了依赖的域行为的流速和脂质混合分数。膜中胆固醇的比例影响这些域的行为。接下来，我们研究了流动下畴的生长。我们讨论了这些趋势的机制，考虑到相分离的自由能，并再现了实验结果的数值模拟。这些发现可能会导致更好地了解非平衡情况下的膜的动力学性质和细胞力学转导的生物物理机制。
The dynamical behaviour of lateral domains on phase-separated lipid vesicles under external flow is reported. A microfluidic chamber was used for the immobilization of vesicles and the application of shear. Microscopic observation revealed that domains tended to be localized at the vortex center and to exhibit a stripe morphology as the flow speed increased. We clarified the dependency of domain behaviors on the flow speed and lipid mixing fraction. The cholesterol ratio in the membrane affected these domain behaviors. Next, we investigated the growth of domains under flow. We discuss the mechanism of these trends by considering the free energy of phase separation, and reproduce the experimental results by numerical simulations. These findings may lead to a better understanding of the dynamical properties of the membrane under nonequilibrium situations and the biophysical mechanism of cellular mechanotransduction.