Molecular cloning, tissue distribution and function analysis of duck TLR7
Molecular cloning, tissue distribution and function analysis of duck TLR7
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鸭TLR7的分子克隆、组织分布及功能分析
DOI：
10.1080/10495398.2020.1784186
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发表时间：
2020-06-30
影响因子：
3.7
通讯作者：
Lu, Lizhi
中科院分区：
文献类型：
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作者：
Gu, Tiantian;Li, Guoqin;Lu, Lizhi
Toll样受体（TLRs）在检测病原体相关分子模式（PAMPs）中发挥重要作用。在TLR中，TLR 7参与抗病毒化合物和单链RNA的识别。本研究旨在探讨禽流感病毒天然宿主鸭TLR 7的结构和功能。首先，采用RT-PCR和RACE技术获得绍兴蛋鸭TLR 7（duTLR 7）的全长cDNA。该基因由38 bp的5 '非翻译区（UTR）、187 bp的3 '非翻译区（UTR）和3270 bp的开放阅读框组成，编码1089个氨基酸。DuTLR 7与其他脊椎动物TLR 7基因具有高度的同源性。在健康鸭中，duTLR 7转录本在不同组织中广泛表达，在肝脏、肾脏和胸腺中表达水平较高。R848刺激可以诱导duTLR 7的最高相对转录水平。此外，通过用poly（I：C）刺激过表达duTLR 7显著促进IFN-β、NF-κ B、IRF 7、TRIF、Mx、STAT 1和STAT 2的表达。综上所述，这些结果表明TLR 7可能在鸭的先天免疫应答中发挥重要作用。
Toll-like receptors (TLRs) play an important role in detecting pathogen-associated molecular patterns (PAMPs). Among the TLRs, TLR7 is involved in the recognition of antiviral compounds and single-stranded RNA. This study was designed to explore the structure and function of TLR7 in duck (Anas platyrhynchos), a natural host for avian influenza virus. Firstly, the full-length cDNA of Shaoxing egg-laying duck TLR7 (duTLR7) was obtained using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). It consisted of 38 base pairs (bp) 5 '-untranslated region (UTR), 187 bp 3 '-UTR, and 3270 bp open reading frame that encodes a single protein of 1089 amino acid residues. DuTLR7 shares high identity with TLR7 genes from other vertebrates. In healthy ducks, duTLR7 transcripts were broadly expressed in different tissues, with higher expression levels in the liver, kidney, and thymus. The highest relative transcript level of duTLR7 could be induced with R848 stimulation. In addition, overexpression of duTLR7 by stimulating with poly(I:C) significantly promoted IFN-beta, NF-kappa B, IRF7, TRIF, Mx, STAT1 and STAT2 expressions. Taken together, these results suggest that TLR7 may play an important role in the innate immune response of ducks.