ROLE OF EXONUCLEASE AND BETA PROTEIN OF PHAGE LAMBDA IN GENETIC RECOMBINATION .5. RECOMBINATION OF LAMBDA DNA IN-VITRO - (SINGLE-STRAND ASSIMILATION/MECHANISM OF ARREST)
ROLE OF EXONUCLEASE AND BETA PROTEIN OF PHAGE LAMBDA IN GENETIC RECOMBINATION .5. RECOMBINATION OF LAMBDA DNA IN-VITRO - (SINGLE-STRAND ASSIMILATION/MECHANISM OF ARREST)
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DOI：
10.1073/pnas.68.7.1639
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
RADDING, CM
中科院分区：
文献类型：
--
作者：
CASSUTO, E;LASH, T;RADDING, CM
λ核酸外切酶和多核苷酸连接酶对冗余连接分子的顺序作用足以产生完整的多核苷酸链和热稳定的、具有生物活性的λ DNA分子，而单独的连接酶的作用是不够的。这些结果（a）证实了先前描述的单链同化机制，包括一种辅助机制，当一条冗余链被完全同化时，λ核酸外切酶的进一步作用被阻止，（B）代表了对双亲复合体（突触）形成后遗传重组步骤的模拟。假设λ核酸外切酶催化协同反应，包括暴露互补序列、形成异源双链体区域和消除冗余分支。
The sequential action of λ exonuclease and polynucleotide ligase upon redundant joint molecules is sufficient to produce intact polynucleotide chains and heat-stable, biologically active molecules of λ DNA, whereas the action of ligase alone is insufficient. These results (a) confirm the previously described mechanism of single-strand assimilation, including a subsidiary mechanism by which the further action of λ exonuclease is arrested when a redundant strand is completely assimilated, and (b) represent a simulation of the steps in genetic recombination that follow the formation of biparental complexes (synapsis). λ exonuclease is postulated to catalyze a concerted reaction that includes exposure of complementary sequences, formation of heteroduplex regions, and elimination of redundant branches.