Antiproliferative effects of natural human tumor necrosis factor-alpha, interferon-alpha, and interferon-gamma on human pancreatic carcinoma cell lines.
Antiproliferative effects of natural human tumor necrosis factor-alpha, interferon-alpha, and interferon-gamma on human pancreatic carcinoma cell lines.
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天然人肿瘤坏死因子-α、干扰素-α和干扰素-γ对人胰腺癌细胞系的抗增殖作用。
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
K. Orita
中科院分区：
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作者：
N. Matsubara;S. Fuchimoto;K. Orita
在人胰腺癌细胞系中研究了天然人肿瘤坏死因子-α（nHuTNF-α）、天然人干扰素-α（nHuIFN-α）和天然人干扰素-γ（nHuIFN-γ）的抗增殖作用。HuP-T3、HuP-T4和BxPC-3癌细胞在用nHuTNF-α处理后表现出轻度生长抑制。HuP-T3、HuP-T4、MIA-PaCa-2和BxPC-3细胞在用nHuIFN-α处理后表现出轻度或显著的生长抑制。与nHuIFN-γ孵育引起HuP-T4和BxPC-3细胞的显著生长抑制，而HuP-T1、HuP-T3和MIA-PaCa-2细胞在相同剂量的nHuIFN-γ下仅显示轻度生长抑制。与它们作为单一药剂对HuP-T1、HuP-T3和MIA-PaCa-2细胞的作用相比，组合的nHuTNF-α和nHuIFN-α（1：1）表现出显著的协同作用。在五种胰腺癌细胞系中的四种（HuP-T1、HuP-T3、HuP-T4和MIA-PaCa-2细胞系）中，与这些细胞因子单独相比，nHuTNF-α和nHuIFN-γ（100：1）的组合也显示出显著的协同效应。通过使用细胞因子的组合所带来的功效的显著增加可能使未来胰腺癌患者的治疗得到一些改善。
The antiproliferative effects of natural human tumor necrosis factor-alpha (nHuTNF-alpha), natural human interferon-alpha (nHuIFN-alpha), and natural human interferon-gamma (nHuIFN-gamma) were investigated in human pancreatic carcinoma cell lines. HuP-T3, HuP-T4, and BxPC-3 carcinoma cells exhibited mild growth inhibition after treatment with nHuTNF-alpha. HuP-T3, HuP-T4, MIA-PaCa-2, and BxPC-3 cells exhibited mild or marked growth inhibition after treatment with nHuIFN-alpha. Incubation with nHuIFN-gamma caused marked growth inhibition of HuP-T4 and BxPC-3 cells, whereas HuP-T1, HuP-T3, and MIA-PaCa-2 cells showed only mild growth inhibition with the same dose of nHuIFN-gamma. Combined nHuTNF-alpha and nHuIFN-alpha (1:1) demonstrated marked synergism in comparison with their effects as single agents on HuP-T1, HuP-T3, and MIA-PaCa-2 cells. The combination of nHuTNF-alpha and nHuIFN-gamma (100:1) also demonstrated a marked synergistic effect in comparison with these cytokines alone in four out of five pancreatic carcinoma cell lines (HuP-T1, HuP-T3, HuP-T4, and MIA-PaCa-2 cell lines). The marked increase in efficacy brought about by using combinations of cytokines may enable some improvement in the treatment of pancreatic carcinoma patients in the future.