Analysis of lipid composition of isolated human sebaceous gland homogenates after incubation with cutaneous bacteria. Thin-layer chromatography.
Analysis of lipid composition of isolated human sebaceous gland homogenates after incubation with cutaneous bacteria. Thin-layer chromatography.
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DOI：
10.1111/1523-1747.ep12512337
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发表时间：
1975-06
期刊：
影响因子：
--
通讯作者：
S. M. Puhvel;R. M. Reisner;M. Sakamoto
中科院分区：
文献类型：
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作者：
S. M. Puhvel;R. M. Reisner;M. Sakamoto
分析了特定种类的皮肤细菌对离体人皮脂腺脂质的影响。将分离的解剖皮脂腺合并、均质化并灭菌，然后掺入蛋白胨-酵母提取物培养基中，并用作痤疮丙酸杆菌、颗粒丙酸杆菌和表皮葡萄球菌亚组II生长的底物。在细菌生长之前和之后通过薄层色谱法分析皮脂脂质。细菌对皮脂腺脂质组成的最显著影响是对皮脂甘油三酯的水解。水解程度因菌株而异，但痤疮丙酸杆菌和颗粒丙酸杆菌的水解程度最完全。葡萄球菌在pH 4.5下不能有效水解皮脂甘油三酯，尽管当培养基的pH升高至pH 6.4时，一些葡萄球菌菌株在将皮脂甘油三酯水解为游离脂肪酸方面与丙酸杆菌一样有效。因此，酸度的微小变化可能在控制葡萄球菌对皮肤的脂解活性方面起重要作用。细菌作用对皮脂腺脂质的另一个影响是将皮脂胆固醇酯化为胆固醇酯。因此，在评估正常和疾病状态下皮肤胆固醇和皮肤表面脂质中胆固醇酯变化的研究时必须考虑细菌的作用。
The effects of specific species of skin bacteria on human sebaceous gland lipids in vitro were analyzed. Isolated dissected sebaceous glands were pooled, homogenized, and sterilized, then incorporated into peptone-yeast extract medium and used as substrate for growth of Propionibacterium acnes, P. granulosum, and Staphylococcus epidermidis subgroup II. The sebaceous lipids were analyzed by thin-layer chromatography before and after bacterial growth. The most striking effect of bacteria on sebaceous gland lipid composition was the hydrolysis of sebaceous triglycerides. The degree of hydrolysis varied with bacterial strain but was most complete with P. acnes and P. granulosum. Staphylococci were not effective in hydrolyzing sebaceous triglycerides at pH 4.5 although, when the pH of the medium was raised to pH 6.4, some strains of staphylococci were as effective as the propionibacteria in hydrolyzing sebaceous triglycerides to free fatty acids. Thus minor changes in acidity may play asignificant role in controlling the lipolytic activity of staphylococci on skin. Another effect of bacterial action on sebaceous gland lipids was the esterification of sebaceous cholesterol to cholesteryl esters. Thus, bacterial action must be taken into account in evaluating studies of alterations in cutaneous cholesterol and cholesteryl esters in skin surface lipids in normal and disease states.