Molecular analysis of three Aeromonas hydrophila AH-3 (serotype 034) lipopolysaccharide core biosynthesis gene clusters
Molecular analysis of three Aeromonas hydrophila AH-3 (serotype 034) lipopolysaccharide core biosynthesis gene clusters
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DOI：
10.1128/jb.01874-07
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发表时间：
2008-05-01
影响因子：
3.2
通讯作者：
Tomas, Juan M.
中科院分区：
文献类型：
--
作者：
Jimenez, Natalia;Canals, Rocio;Tomas, Juan M.
By the isolation of three different Aeromonas hydrophila strain AH-3 (serotype 034) mutants with an altered lipopolysaccharide (LPS) migration in gels, three genomic regions encompassing LPS core biosynthesis genes were identified and characterized. When possible, mutants were constructed using each gene from the three regions, containing seven, four, and two genes (regions I to 3, respectively). The mutant LPS core structures were elucidated by using mass spectrometry, methylation analysis, and comparison with the full core structure of an O-antigen-lacking AH-3 mutant previously established by us. Combining the gene sequence and complementation test data with the structural data and phenotypic characterization of the mutant LPSs enabled a presumptive assignment of all LPS core biosynthesis gene functions in A. hydrophila AH-3. The three regions and the genes contained are in complete agreement with the recently sequenced genome of A. hydrophila ATCC 7966. The functions of the A. hydrophila genes waaC in region 3 and waaF in region 2 were completely established, allowing the genome annotations of the two heptosyl transferase products not previously assigned. Having the functions of all genes involved with the LPS core biosynthesis and most corresponding single-gene mutants now allows experimental work on the role of the LPS core in the virulence of A. hydrophila.
