Dose optimisation of PTEN inhibitor, bpV (HOpic), and SCF for the in-vitro activation of sheep primordial follicles
Dose optimisation of PTEN inhibitor, bpV (HOpic), and SCF for the in-vitro activation of sheep primordial follicles
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DOI：
10.1080/08977194.2019.1680661
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发表时间：
2019-10-22
期刊：
影响因子：
1.8
通讯作者：
Alikhani, Mehdi
中科院分区：
文献类型：
--
作者：
Adib, Samane;Valojerdi, Mojtaba Rezazadeh;Alikhani, Mehdi
原始卵泡的体外发育对于提高哺乳动物的生育能力和野生动物保护至关重要。本研究旨在优化bpV（HOpic）和干细胞因子（SCF）体外激活绵羊原始卵泡的有效剂量。为此，绵羊卵巢皮质用bpV（1.5、15和150 μ M）和SCF（50和100 ng/ml）处理。卵泡计数表明，15 μ M bpV和100 ng/ml SCF显著增加了正常初级卵泡（p < 0.05）。此外，观察到P53和PTEN的显著下调，以及PI 3 K的表达增加。当用选定剂量的bpV和SCF共同处理碎片组织时，体外成熟更加明显。总之，15 μ M bpV和100 ng/ml SCF的组合是绵羊卵巢碎片中原始卵泡激活和存活的最有效治疗策略。
The in-vitro development of primordial follicles is critical for improving mammalian fertility and wildlife conservation. This study aimed to optimise the effective doses of bpV (HOpic) and stem cell factor (SCF) for the in-vitro activation of sheep primordial follicles. To do this, sheep ovarian cortex was treated with bpV (1.5, 15, and 150 mu M) and SCF (50 and 100 ng/ml). Follicular count indicated that 15 mu M bpV and 100 ng/ml SCF significantly increased normal primary follicles compared to other groups (p < 0.05). Also, a significant downregulation of P53 and PTEN, as well as the increased expression of PI3K was observed. The in-vitro maturation was more pronounced when the fragmented tissues were co-treated with selected doses of bpV and SCF. In conclusion, the combination of 15 mu M bpV and 100 ng/ml SCF was the most effective treatment strategy for the activation and survival of primordial follicles in sheep ovarian fragments.