Lipopolysaccharide structures in Enterobacteriaceae, Pseudomonas aeruginosa, and Vibrio cholerae are immunologically related to Campylobacter spp
Lipopolysaccharide structures in Enterobacteriaceae, Pseudomonas aeruginosa, and Vibrio cholerae are immunologically related to Campylobacter spp
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肠杆菌科、铜绿假单胞菌和霍乱弧菌中的脂多糖结构与弯曲杆菌属具有免疫相关性
DOI：
10.1128/iai.51.1.204-208.1986
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发表时间：
1986
影响因子：
3.1
通讯作者：
M. Blaser
中科院分区：
文献类型：
--
作者：
G. Perez;J. Hopkins;M. Blaser
要确定是否弯曲杆菌属的细菌的脂多糖（LPS）的结构是免疫相关的其他11个革兰氏阴性菌，我们免疫印迹聚丙烯酰胺凝胶的LPS这些菌株与免疫兔血清提出对6个空肠弯曲杆菌菌株和两个弯曲杆菌胎儿菌株。研究的LPS来自沙门氏菌野生型和Ra至Re突变体、伤寒沙门氏菌、大肠杆菌、小肠结肠炎耶尔森氏菌、霍乱弧菌和铜绿假单胞菌。11个LPS制剂中没有一个被8种抗血清识别，但是每个弯曲杆菌菌株的抗血清识别一些LPS制剂的核心决定簇。抗血清针对最敏感的血清C。jejuni菌株79-193是唯一识别粗糙沙门氏菌突变体核心区域的抗血清样品。在匡威的实验中，当从5个弯曲杆菌菌株的LPS制剂与沙门氏菌脂质A的抗血清进行印迹，每个核心结构的识别显示，从酶联免疫吸附试验的数据证实了这一结果。相反，鼠伤寒沙门氏菌Re LPS的抗血清显示无反应性。我们的结论是，弯曲杆菌菌株的LPS与其他几种革兰氏阴性菌的LPS的核心区域共享脂质A抗原决定簇。
To determine whether lipopolysaccharide (LPS) structures of Campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the LPS of these strains with immune rabbit serum raised against six Campylobacter jejuni strains and two Campylobacter fetus strains. The LPS studied were from Salmonella minnesota wild type and Ra to Re mutants, Salmonella typhi, Escherichia coli, Yersinia enterocolitica, Vibrio cholerae, and Pseudomonas aeruginosa. None of the 11 LPS preparations was recognized by the eight antisera, but antisera to each of the Campylobacter strains recognized core determinants of some LPS preparations. Antiserum directed against the most serum-sensitive C. jejuni strain, 79-193, was the only antiserum sample that recognized core regions of the rough Salmonella mutants. In converse experiments, when LPS preparations from five Campylobacter strains were blotted with antiserum to Salmonella lipid A, recognition of core structures of each was shown; data from an enzyme-linked immunosorbent assay confirmed this result. In contrast, antiserum to Salmonella typhimurium Re LPS showed no reactivity. We conclude that LPS of Campylobacter strains share lipid A antigenic determinants with the core region of LPS of several other gram-negative organisms.