The effect of alkaline pretreatment on the biochemical characteristics and fibril-forming abilities of types I and II collagen extracted from bester sturgeon by-products
The effect of alkaline pretreatment on the biochemical characteristics and fibril-forming abilities of types I and II collagen extracted from bester sturgeon by-products
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DOI：
10.1016/j.ijbiomac.2019.03.091
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发表时间：
2019-06-15
影响因子：
8.2
通讯作者：
Takagi, Yasuaki
中科院分区：
文献类型：
--
作者：
Meng, Dawei;Tanaka, Hiroyuki;Takagi, Yasuaki
非哺乳动物胶原蛋白在工业和生物医学上的应用越来越受到人们的关注。因此，我们评估了从水产养殖鲟鱼胶原蛋白的提取条件和生化特性。从鲟鱼下脚料的鱼皮和脊索中分别提取了胃蛋白酶可溶性Ⅰ型和Ⅱ型胶原蛋白，得率分别为63.9 ± 0.19%和35.5 ± 0.68%。通过皮肤和脊索的碱性预处理提高了胶原蛋白提取效率（需要更少的提取循环），但I型的最终产率降至56.2 +/- 0.84%，II型降至31.8 +/- 1.13%。碱性预处理不影响两种类型的胶原蛋白的热稳定性或三螺旋结构。I型和II型胶原在不同条件下在体外形成重新组装的原纤维结构。碱性预处理减缓了I型胶原纤维的形成，并特异性地抑制了粗纤维束结构的形成。相反，碱性预处理并没有改变II型胶原纤维的形成。综上所述，碱预处理鲟鱼皮和脊索是一种有效的方法，可以在不改变I型和II型胶原蛋白生化特性的前提下，加快其胶原蛋白的提取过程。然而，它降低了两种胶原蛋白的产量，并特别改变了I型胶原蛋白的原纤维形成能力。(C)2019爱思唯尔B. V.保留所有权利。
Non-mammalian collagens have attracted increasing attention for industrial and biomedical use. We have therefore evaluated extraction conditions and the biochemical properties of collagens from aquacultured sturgeon. Pepsin-soluble type I and type II collagen were respectively extracted from the skin and notochord of bester sturgeon by-products, with yields of 63.9 +/- 0.19% and 35.5 +/- 0.68%. Collagen extraction efficiency was improved by an alkaline pretreatment of the skin and notochord (fewer extraction cycles were required), but the final yields decreased to 56.2 +/- 0.84% for type I and 31.8 +/- 1.13% for type II. Alkaline pretreatment did not affect the thermal stability or triple-helical structure of both types of collagen. Types I and II collagen formed re-assembled fibril structures in vitro, under different conditions. Alkaline pretreatment slowed down the formation of type I collagen fibrils and specifically inhibited the formation of thick fibril-bundle structures. In contrast, alkaline pretreatment did not change type II collagen fibril formation. In conclusion, alkaline pretreatment of sturgeon skin and notochord is an effective method to accelerate collagen extraction process of types I and II collagen without changing their biochemical properties. However, it decreases the yield of both collagens and specifically changes the fibril-forming ability of type I collagen. (C) 2019 Elsevier B.V. All rights reserved.