Insight on Genes Affecting Tuber Development in Potato upon Potato spindle tuber viroid (PSTVd) Infection.
Insight on Genes Affecting Tuber Development in Potato upon Potato spindle tuber viroid (PSTVd) Infection.
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DOI：
10.1371/journal.pone.0150711
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hornyik C
中科院分区：
文献类型：
--
作者：
Katsarou K;Wu Y;Zhang R;Bonar N;Morris J;Hedley PE;Bryan GJ;Kalantidis K;Hornyik C
马铃薯 (Solanum tuberosum L) 是马铃薯纺锤形块茎类病毒 (PSTVd) 的天然宿主，该病毒可导致发育中的植物出现特征性症状，包括发育迟缓表型以及叶子和块茎变形。 PSTVd 是 Pospiviroidae 科的模式种，可以在细胞核中复制并在整个植物中系统移动。目前尚不清楚类病毒如何影响宿主基因以成功入侵，以及哪些基因在感染后表现出表达水平的改变。我们在这项研究中的主要重点是鉴定可以影响块茎形成的基因，因为类病毒感染可以强烈影响块茎发育，特别是块茎形状。在这项研究中，我们采用大规模方法来鉴定马铃薯中的差异表达基因。我们已经鉴定出与防御、压力和糖代谢相关的基因在感染时表达水平发生改变。此外，激素途径相关基因显示出显着的上调或下调。鉴定并验证了 DWARF1/DIMINUTO、赤霉素 7-氧化酶和 BEL5 转录本，在类病毒感染的组织中显示差异表达。我们的研究表明，赤霉素和油菜素类固醇途径可能在 PSTVd 感染后块茎发育中发挥作用。
Potato (Solanum tuberosum L) is a natural host of Potato spindle tuber viroid (PSTVd) which can cause characteristic symptoms on developing plants including stunting phenotype and distortion of leaves and tubers. PSTVd is the type species of the family Pospiviroidae, and can replicate in the nucleus and move systemically throughout the plant. It is not well understood how the viroid can affect host genes for successful invasion and which genes show altered expression levels upon infection. Our primary focus in this study is the identification of genes which can affect tuber formation since viroid infection can strongly influence tuber development and especially tuber shape. In this study, we used a large-scale method to identify differentially expressed genes in potato. We have identified defence, stress and sugar metabolism related genes having altered expression levels upon infection. Additionally, hormone pathway related genes showed significant up- or down-regulation. DWARF1/DIMINUTO, Gibberellin 7-oxidase and BEL5 transcripts were identified and validated showing differential expression in viroid infected tissues. Our study suggests that gibberellin and brassinosteroid pathways have a possible role in tuber development upon PSTVd infection.