Activity-based protein profiling for the functional annotation of enzymes
Activity-based protein profiling for the functional annotation of enzymes
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DOI：
10.1038/nmeth1092
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发表时间：
2007-10-01
期刊：
影响因子：
48
通讯作者：
Cravatt, Benjamin F.
中科院分区：
文献类型：
--
作者：
Barglow, Katherine T.;Cravatt, Benjamin F.
基于活性的蛋白质谱分析（ABPP），使用活性定点化学探针来监测复杂生物系统中的酶功能，正在成为一种强大的后基因组技术。ABPP探针已被开发用于几种酶类别，并已被用于库存酶的活动，在一系列（病理）生理过程中。通过提出具体的例子，我们在这里表明，ABPP为研究人员提供了一套独特的化学工具，开始分配功能的许多未知的酶，填充真核和原核蛋白质组。
Activity-based protein profiling ( ABPP), the use of active site-directed chemical probes to monitor enzyme function in complex biological systems, is emerging as a powerful post-genomic technology. ABPP probes have been developed for several enzyme classes and have been used to inventory enzyme activities en masse for a range of ( patho) physiological processes. By presenting specific examples, we show here that ABPP provides researchers with a distinctive set of chemical tools to embark on the assignment of functions to many of the uncharacterized enzymes that populate eukaryotic and prokaryotic proteomes.